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Sci Rep. 2018 Jan 12;8(1):561. doi: 10.1038/s41598-017-18973-9.

Inactivated rotavirus vaccine by parenteral administration induces mucosal immunity in mice.

Author information

1
Division of Viral Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA.
2
School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
3
Division of Viral Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA. bxj4@cdc.gov.

Abstract

To improve the safety and efficacy of oral rotavirus vaccines, we developed an inactivated rotavirus vaccine (IRV) for parenteral administration. Since it remains unknown whether parenteral vaccination can induce mucosal immunity, we performed a comprehensive assessment of immune responses to IRV in mice with an adjuvant-free dissolving polymer MN patch or by alum-adjuvanted IM injection. We demonstrated that IRV induced the expression of the gut homing receptor LPAM-1 on T and B cells in spleen and mLN of vaccinated mice. MN patch IRV vaccination induced a slight Th1 phenotype while IM vaccination induced a balanced Th1/Th2 phenotype. In addition, a dose-sparing effect was seen for rotavirus-specific serum IgG and neutralizing activity for both vaccination routes. Our study is the first to show that parenterally administered IRV can induce mucosal immunity in the gut, in addition to strong serum antibody response, and is a promising candidate vaccine in achieving global immunization against rotavirus.

PMID:
29330512
PMCID:
PMC5766576
DOI:
10.1038/s41598-017-18973-9
[Indexed for MEDLINE]
Free PMC Article

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