(A) Maximum likelihood phylogeny based on an amino acid alignment of tetrapod Arc, fly dArc1, and Gag sequences from related Ty3/gypsy retrotransposons. Schematics of Gag-only Arc genes and Ty3/gypsy elements are included to the right of the tree. In lineages without Arc genes, the most closely related sequences to Arc are Gag-pol poly-proteins flanked by long terminal repeats (LTRs) as expected in bona fide Ty3/gypsy retrotransposons.
(B) (top) Representative negative stain EM images of full-length purified rat Arc (prArc) protein (1mg/mL, 42,000x). (i–iv) Magnified view of boxed particles. Scale bars=30nm. Representative cryo-EM images of prArc (2mg/mL, 62,000x). (v–vii) Magnified images of Arc capsids showing the double-layered capsid shell. Scale bars=30nm. (bottom) Dynamic light scattering analysis of prArc capsids. The weighted size distribution profile is represented as a histogram of the number of particles.
(C) Schematic of Arc protein with the predicted matrix (MA) (orange), CA-NTD (green), and CA-CTD (blue) domains. Also depicted: ΔCTD deletion mutant and the CA domain constructs. Representative negative stain EM images of purified GST, prArc, the Drosophila Arc homologue dArc1, prArc-ΔCTD, and CA-prArc (all 1mg/mL, 20,000x). Inset scale bars=50nm. (bottom) Quantification of capsid formation. Fully formed capsids include spherical particles that are between 20–60nm and have clear double shells, while partially formed capsids do not have clear double shells (scale bars=100nm). Data is the average of 3 independent experiments±SEM using 3 different prArc preparations. ***p<0.001, two-way ANOVA with post hoc t-tests.
(D) (top) To determine properties of Arc capsid stability, prArc was exchanged into buffers with increasing molar concentrations of salt and examined by negative stain EM. Arc capsids were counted manually and quantified in each buffer condition at a protein concentration of 1.5mg/mL. Data is the average of 3 independent experiments±SEM using different prArc preparations. **p<0.01, Student’s t-test. (bottom) Representative EM images of prArc under 0M NaCl and 0.5M NaPO₄ conditions.

(A) (left) qRT-PCR of Arc mRNA and the bacterial mRNA asnA from prArc. (right) qRT-PCR of Arc and asnA mRNA from total bacteria lysate. Data presented as the mean±SEM normalized to the average of the asnA group (Student’s t-test, n=3 independent protein preparations, *p<0.05).
(B) Protein preparations were treated with or without RNase A for 15 min and qRT-PCR was performed. RNase treatment did not affect Arc and asnA mRNA levels (paired t-test, n=5 independent protein samples), but significantly degraded exogenous/free GFP mRNA (paired t-test, n=3 independent samples, *p<0.05). Data presented as ±SEM normalized to the average of the untreated group.
(C) (top) Representative Western blots of Arc protein that was immunoprecipitated (IP) from WT mouse cortical tissue using an Arc or IgG antibody. Input (I)=10% total lysate. (bottom, left) Quantification of Arc protein IP, showing significant enrichment of Arc protein using an Arc antibody. (bottom, right) qRT-PCR was performed on the eluted fractions from the IP. Arc mRNA was specifically pulled down in the IP (two-way ANOVA with repeated measures and Sidak’s multiple comparisons: Arc+Arc vs. Arc+IgG, p=0.01; Arc+Arc vs. GAPDH+Arc, p=0.013; Arc+Arc vs. GAPDH+IgG, p=0.011). Data presented as the mean±SEM normalized to the average of the IgG group.
(D) qRT-PCR of Arc mRNA from prArc and prArc(RNA−). There was significantly less Arc mRNA in the prArc(RNA−) preparations. Presented as the mean±SEM normalized to the average of the prArc group (Student’s t-test, n=3 independent samples, *p=0.05).
(E) (left) Representative negative stain EM images of prArc, prArc(RNA−), and prArc(RNA−) incubated with 7.3% (w/w) GFP mRNA at RT for 2h (0.25mg/mL, 15,000x). Fully formed capsids are indicated by red arrows (scale bars=100nm). (right) Capsids were quantified as in . Data is presented as the average of 6 images from each condition ±SEM. ***p<0.001, unpaired t-test.

(A) HEK cells in 10-cm dishes were transfected with full-length rat WT myc-Arc and media collected 24h later. Representative Western blots (n=3 independent experiments) show Arc protein in total cell lysates (cells) and the EV fraction purified from cell media in Arc transfected (+) and untransfected (−) cells. ALIX was used as an EV fraction marker. Ponceau stain was used to visualize the total amount of protein in each lane.
(B) HEK293 cells were transfected with myc-Arc-WT or myc-Arc-ΔCTD and media collected 24h later. Representative Western blots (n=3 independent experiments) show Arc protein in total cell lysates (cells) and the EV fraction from cell media. Arc levels in the EV fraction were normalized to Arc protein levels in the cell lysate for each experiment and data is presented normalized to WT levels (n=3). *p<0.05, Student’s t-test.
(C) HEK EV fractions were untreated (control) or treated with RNase (n=6 independent cultures) prior to RNA extraction. qRT-PCR was used to measure Arc mRNA levels and data is presented as the mean±SEM normalized to the average of the untreated group. Paired t-test.
(D) Media was harvested from DIV15 cultured cortical neurons obtained from WT and Arc KO mice after 24h incubation and the EV fraction was purified from collected media. Blots indicate levels of Arc, ALIX, and actin from supernatant (S)/soluble fraction and pellet (P)/insoluble fraction for total cellular lysate (cells). (S)/last wash of the ultracentrifugation purification protocol and final pellet (P)/EV fraction for purified EV fraction (EVs). 2.5% of S and P were loaded for cellular lysates. 5% of S and P were loaded for the EV fraction.
(E) RT-PCR using Arc and GAPDH primers was performed on WT or KO mouse cortical tissue, mouse cortical DIV15 WT or KO neurons (cells), and EVs purified from media collected from WT or KO cultured neurons. Arc mRNA was present in all three preparations, while GAPDH mRNA was absent from EVs.
(F) (top) Immunogold labeling for Arc in EVs obtained from the same Arc KO or WT cultured neuronal media in (D). Red arrow indicates a 10nm immunogold particle (20,000x). (bottom) Quantification of EVs (vesicular structures <100nm) that were Arc-positive±SEM, using immunogold labeling (n=3 independent experiments/EV preparations). ***p<0.001, Student’s t-test.

(A) Donor HEK cells in 10-cm dishes were transfected with GFP-Arc, myc-Arc, or nuclear GFP (nucGFP) for 6h. Culture media containing plasmid DNA and transfection reagents was then removed and replaced with fresh culture media. 18h later, this media was removed and used to replace media on naïve recipient HEK cells on coverslips in 12-well plates. 24h later, these cells were fixed and combined FISH for Arc mRNA and ICC for Arc protein was performed. (left) Representative images of HEK cells grown on coverslips and transfected with the same protocol as in 10-cm dishes, showing Arc protein (ICC) and Arc mRNA (FISH). (Right) Representative images of recipient HEK cells showing Arc mRNA and protein were present in cells that received media from GFP-Arc- and myc-Arc-transfected cells, but not nucGFP-transfected cells. Scale bar=20μm. Representative of 7 independent experiments and cultures. (B) Donor HEK cells in 10-cm dishes were transfected as in (A) with membrane GFP (mGFP), myc-Arc, or both constructs together. The media was replaced after 6h, and 18h later, transferred to naïve recipient HEK cells in 12-well plates. 24h later, cells were fixed and combined FISH/ICC for GFP mRNA and Arc protein was performed. (left) Representative images of transfected HEK cells grown on coverslips, showing mGFP fluorescence, Arc protein and GFP mRNA. (right) Representative images of recipient HEK cells that show co-transfer of GFP protein and mRNA with Arc protein. No GFP transfer was observed in the mGFP only group. Scale bar=20μm. Representative of 3 independent experiments and cultures.

(A) Representative images of Arc ICC from DIV15 cultured hippocampal Arc KO neurons treated for 1 or 4h with 4μg prArc or WT control neurons. prArc-treated neurons showed increased dendritic Arc levels in untreated KO neurons. (B) Neurons were treated as in (A); representative images of Arc mRNA (FISH) are shown. 4h of prArc treatment significantly increased dendritic Arc mRNA levels. (C) Representative images of Arc ICC from DIV15 cultured hippocampal KO neurons treated with 4μg prArc, prArc-ΔCTD, or CA-prArc for 4h. KO neurons treated with prArc-ΔCTD and CA-prArc showed lower levels of Arc protein than prArc. (D) Neurons were treated as in (C); representative images of Arc mRNA are shown. Neurons treated with prArc-ΔCTD and CA-prArc showed lower levels of Arc mRNA than prArc. Dendritic segments boxed in white are shown magnified beneath each corresponding image. 30-μm segments of two dendrites/neuron were analyzed for integrated density measurements in all groups (n=10 neurons). Arc mRNA and Arc protein levels were normalized to untreated KO neurons and displayed as fold-change±SEM. Student’s t-test: *p<0.05. **p<0.01. ***p<0.001. Scale bars=10μm. Images are false-colored with the Smart LUT from ImageJ. All data are representative of 3–7 independent experiments using different protein preparations and cultures.

(A) Representative images of Arc ICC from DIV15 cultured hippocampal Arc KO neurons treated for 1 or 4h with 10μg of the EV fraction prepared from 10-cm dishes of DIV15 high-density cortical WT or Arc KO neurons. 1 and 4h treatment with KO EVs did not increase dendritic Arc levels, whereas 1 and 4h of treatment with WT EVs significantly increased dendritic Arc protein levels. (B) Neurons were treated as in (A); representative images of Arc mRNA (FISH) are shown. 1 and 4h treatment with KO EV did not increase dendritic Arc mRNA levels. 1h of treatment with WT EV did not significantly increase dendritic Arc levels, while 4h increased dendritic Arc mRNA levels. 30-μm segments of two dendrites/neuron were analyzed for integrated density measurements in all groups (n=10 neurons). Arc mRNA and Arc protein levels were normalized to untreated KO neurons and displayed as fold-change±SEM. Student’s t-test: *p<0.05. **p<0.01. ***p<0.001. Scale bars=10μm. Representative of 6 independent experiments using different EV preparations and cultures.

(A) Representative images of Arc ICC from DIV15 cultured hippocampal Arc KO neurons treated for 4h with 4μg prArc. To induce translation of Arc mRNA, 30 min prior to fixation, neurons were treated with the mGluR1/5 agonist DHPG (100μM) for 5 min and then washed out. 1h prior to fixation, a subset of neurons was pretreated with cycloheximide (CHX; 180μM) to block protein translation. prArc significantly increased dendritic Arc expression in KO neurons and DHPG treatment further increased dendritic Arc levels, which was blocked by pretreatment with CHX. DHPG had no effect on untreated KO neurons. (B) Representative images of Arc ICC from DIV15 hippocampal Arc KO neurons treated for 4h with 10μg of the EV fraction prepared from 10-cm dishes of DIV15 high density cortical WT or Arc KO neurons. A subset of neurons was treated with DHPG and CHX as in (A). WT EVs significantly increased dendritic Arc expression in KO neurons, while KO EVs had no effect. DHPG treatment had no effect on dendritic Arc expression in untreated KO neurons or KO EV-treated KO neurons. However, DHPG treatment significantly increased dendritic Arc levels in WT EV-treated KO neurons, which was blocked by pretreatment with CHX. 30-μm segments of two dendrites/neuron were analyzed for integrated density measurements in all groups (n=10 neurons). Arc mRNA and Arc protein levels were normalized to untreated KO neurons and displayed as fold-change±SEM. Student’s t-test: *p<0.05. **p<0.01. ***p<0.001. Scale bars=10μm. Representative of 3 independent experiments using different EV/protein preparations and cultures.