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J Vet Diagn Invest. 2018 May;30(3):406-412. doi: 10.1177/1040638717753966. Epub 2018 Jan 12.

Linear epitopes in African swine fever virus p72 recognized by monoclonal antibodies prepared against baculovirus-expressed antigen.

Heimerman ME1,2,3,4,5, Murgia MV1,2,3,4,5, Wu P1,2,3,4,5, Lowe AD1,2,3,4,5, Jia W1,2,3,4,5, Rowland RR1,2,3,4,5.

Author information

1
Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS (Heimerman, Murgia, Rowland).
2
Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, Animal and Plant Health Inspection Services, U.S. Department of Agriculture, Plum Island Animal Disease Center, New York, NY (Wu, Lowe, Jia).
3
Current addresses: National Emerging Infectious Disease Laboratories, Boston, MA (Lowe).
4
Thermo Fisher Scientific, Lenexa, KS (Heimerman).
5
Purdue University, West Lafayette, IN (Murgia).

Abstract

Protein p72 is the major capsid protein of African swine fever virus (ASFV) and is an important target for test and vaccine development. Monoclonal antibodies (mAbs) were prepared against a recombinant antigenic fragment, from amino acid (aa) 20-303, expressed in baculovirus. A total of 29 mAbs were recovered and tested by immunofluorescent antibody (IFA) staining on ASFV Lisbon-infected Vero cells. Six antibodies were IFA-positive and selected for further characterization. Epitope mapping was performed against overlapping polypeptides expressed in E. coli and oligopeptides. Based on oligopeptide recognition, the mAbs were divided into 4 groups: mAb 85 (aa 165-171); mAbs 65-3 and 6H9-1 (aa 265-280); mAbs 8F7-3 and 23 (aa 280-294); and mAb 4A4 (aa 290-303). All mAbs were located within a highly conserved region in p72. This panel of antibodies provides the opportunity to develop new assays for the detection of ASFV antibody and antigen.

KEYWORDS:

African swine fever virus; monoclonal antibodies; p72

PMID:
29327672
PMCID:
PMC6505808
DOI:
10.1177/1040638717753966
[Indexed for MEDLINE]
Free PMC Article

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