Format

Send to

Choose Destination
Int J Biol Macromol. 2018 May;111:370-378. doi: 10.1016/j.ijbiomac.2018.01.042. Epub 2018 Jan 8.

Dextran hydrogels by crosslinking with amino acid diamines and their viscoelastic properties.

Author information

1
Department of Chemistry, Lehman College of the City University of New York, Bronx, NY 10468, United States; Ph.D. Program in Chemistry, The Graduate Center of the City University of New York, New York, NY 10016, United States; Ph.D. Program in Biochemistry, The Graduate Center of the City University of New York, New York, NY 10016, United States. Electronic address: naphtali.oconnor@lehman.cuny.edu.
2
Department of Chemistry, William Paterson University, 300 Pompton Rd, Wayne, NJ 07470, United States.
3
Department of Chemistry, Lehman College of the City University of New York, Bronx, NY 10468, United States.
4
Department of Chemistry, Lehman College of the City University of New York, Bronx, NY 10468, United States; Department of Biology, Lehman College of the City University of New York, Bronx, NY 10468, United States.
5
Department of Biology, Lehman College of the City University of New York, Bronx, NY 10468, United States.
6
Department of Biology, Lehman College of the City University of New York, Bronx, NY 10468, United States; Biological Sciences Department, Bronx Community College of the City University of New York, Bronx, NY 10453, United States.
7
Department of Chemistry, Lehman College of the City University of New York, Bronx, NY 10468, United States; Ph.D. Program in Chemistry, The Graduate Center of the City University of New York, New York, NY 10016, United States; Ph.D. Program in Biochemistry, The Graduate Center of the City University of New York, New York, NY 10016, United States.
8
Department of Biology, Lehman College of the City University of New York, Bronx, NY 10468, United States; Ph.D. Program in Biology, The Graduate Center of the City University of New York, New York, NY 10016, United States; Ph.D. Program in Biochemistry, The Graduate Center of the City University of New York, New York, NY 10016, United States.

Abstract

Amine functionalized polysaccharide hydrogels such as those based on chitosan are widely examined as biomaterials. Here we set out to develop a facile procedure for developing such hydrogels by crosslinking dextran with amino acid diamines. The dextran-amino acid gels were formed by the addition of the amino acid diamines to a dextran and epichlorohydrin solution once it became homogeneous. This was demonstrated with three amino acid diamines, lysine, lysine methyl ester, and cystine dimethyl ester. Hydrogel networks with albumin entrapped were also demonstrated. These hydrogels were characterized by FTIR, SEM, rotational rheometry, swelling studies and cell biocompatibility analysis. These hydrogels showed the unexpected pH-responsive behavior of greater swelling at more basic pH, similar to that of an anionic hydrogel. This is uncharacteristic for amine functionalized gels as they typically exhibit cationic hydrogel behavior. All hydrogels showed similar biocompatibility to that of dextran crosslinked without amino acids.

KEYWORDS:

Cell biocompatability; Hydrogels; Viscoelastic properties; pH-responsive

PMID:
29325744
PMCID:
PMC5860956
[Available on 2019-05-01]
DOI:
10.1016/j.ijbiomac.2018.01.042
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center