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Zhonghua Gan Zang Bing Za Zhi. 2017 Nov 20;25(11):847-851. doi: 10.3760/cma.j.issn.1007-3418.2017.11.009.

[Characteristics of IgH-CDR3 repertoire of peripheral B cells in a patient with primary biliary cholangitis: a preliminary study using high-throughput sequencing].

[Article in Chinese; Abstract available in Chinese from the publisher]

Author information

1
Clinical Research Center for Autoimmune Liver Disease & Clinical Laboratory Center, Beijing You'an Hospital, Capital Medical University, Beijing 100069, China.
2
National Research Institute for Family Planning, Beijing 100081, China.
3
Department of Liver Disease Immunology, Beijing You'an Hospital, Capital Medical University, Beijing 100069, China.

Abstract

in English, Chinese

Objective: To analyze the characteristics of immunoglobulin heavy chain complementarity-determining region (IgH-CDR3) repertoire of peripheral B cells in a patient with primary biliary cholangitis (PBC) and to investigate the diversity of the immune system. Methods: Arm-PCR was used to amplify the IgH-CDR3 region of circulating B cells isolated from a PBC patient, and high-throughput sequencing was used to analyze the amplified product. The characteristics of immune repertoire were analyzed by bioinformatics. Results: In total, 329219 sequence reads were generated from the sample, with 325540 total CDR3 sequences and 72774 distinct CDR3 sequences, and the D50 of IGH-CDR3 was 7.7. The dominant CDR3 length of the sample was 45 nt (9.6%); the N addition with the highest frequency ranged from 13 to 14 nt (5.25%); the J trimming with the highest frequency was 0 nt (12.7%); the three most frequent V alleles were V4-59 (9.5%), V3-23 (8.1%), and V1-69 (6.4%). Conclusion: The diversity of IgH-CDR3 repertoire is relatively low in this patient with PBC, with several B-cell clonal expansions. The specificity needs to be further verified after increasing the sample size.

KEYWORDS:

B cell receptor; IGH-CDR3 repertoire; Liver cirrhosis, biliary

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