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PLoS One. 2018 Jan 11;13(1):e0190819. doi: 10.1371/journal.pone.0190819. eCollection 2018.

Genetic diversity and recombination of enterovirus G strains in Japanese pigs: High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population.

Author information

1
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
2
Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Tottori, Japan.
3
Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa, Japan.
4
Kenpoku Livestock Hygiene Service Center, Mito, Ibaraki, Japan.
5
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Yanagido, Gifu, Japan.
6
Education and Research Center for Food Animal Health, Gifu University (GeFAH), Gifu, Japan.
7
Center for Highly Advanced Integration of Nano and Life Sciences, Gifu University (G-CHAIN), Gifu, Japan.
8
Laboratory of Small Animal Clinics, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
9
Department of Veterinary Medicine, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
10
Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan.
11
Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America.
12
Department of Bioproduction Science, Ishikawa Prefectural University, Nonoichi, Ishikawa, Japan.

Abstract

To study the genetic diversity of enterovirus G (EV-G) among Japanese pigs, metagenomics sequencing was performed on fecal samples from pigs with or without diarrhea, collected between 2014 and 2016. Fifty-nine EV-G sequences, which were >5,000 nucleotides long, were obtained. By complete VP1 sequence analysis, Japanese EV-G isolates were classified into G1 (17 strains), G2 (four strains), G3 (22 strains), G4 (two strains), G6 (two strains), G9 (six strains), G10 (five strains), and a new genotype (one strain). Remarkably, 16 G1 and one G2 strain identified in diarrheic (23.5%; four strains) or normal (76.5%; 13 strains) fecal samples possessed a papain-like cysteine protease (PL-CP) sequence, which was recently found in the USA and Belgium in the EV-G genome, at the 2C-3A junction site. This paper presents the first report of the high prevalence of viruses carrying PL-CP in the EV-G population. Furthermore, possible inter- and intragenotype recombination events were found among EV-G strains, including G1-PL-CP strains. Our findings may advance the understanding of the molecular epidemiology and genetic evolution of EV-Gs.

PMID:
29324778
PMCID:
PMC5764308
DOI:
10.1371/journal.pone.0190819
[Indexed for MEDLINE]
Free PMC Article

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