Format

Send to

Choose Destination
Methods Mol Biol. 2018;1725:163-176. doi: 10.1007/978-1-4939-7568-6_14.

Quantifying Caspase-1 Activity in Murine Macrophages.

Author information

1
Institute for Molecular Bioscience and IMB Centre for Inflammation and Disease Research, The University of Queensland, St. Lucia, Australia.
2
Institute for Molecular Bioscience and IMB Centre for Inflammation and Disease Research, The University of Queensland, St. Lucia, Australia. K.Schroder@imb.uq.edu.au.

Abstract

The caspase-1 protease is a core component of multiprotein inflammasome complexes, which play a critical role in regulating the secretion of mature, bioactive pro-inflammatory cytokines interleukin (IL)-1β and IL-18. The activity of caspase-1 is often measured indirectly, by monitoring cleavage of cellular caspase-1 substrates, processing of caspase-1 itself, or by quantifying cell death. Here we describe methods for eliciting caspase-1 activity in murine macrophages, via activation of the NLRP3, NAIP/NLRC4 or AIM2 inflammasomes. We then describe a simple fluorogenic assay for directly quantifying cellular caspase-1 activity.

KEYWORDS:

Biochemistry; Inflammasome; Inflammatory caspases; Protease

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center