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Sci Rep. 2018 Jan 10;8(1):350. doi: 10.1038/s41598-017-18582-6.

Uncoupled mitochondria quickly shorten along their long axis to form indented spheroids, instead of rings, in a fission-independent manner.

Author information

1
Division Microscopic and Development Anatomy, Department of Anatomy Kurume University School of Medicine, Kurume, 830-0011, Japan.
2
Dental and Oral Medical Center, Kurume University School of Medicine, Kurume, 830-0011, Japan.
3
Department of Protein Biochemistry, Institute of Life Science, Kurume University, Kurume, 839-0864, Japan.
4
Division Microscopic and Development Anatomy, Department of Anatomy Kurume University School of Medicine, Kurume, 830-0011, Japan. kohta@med.kurume-u.ac.jp.
5
Advanced Imaging Research Center, Kurume University School of Medicine, Kurume, 830-0011, Japan. kohta@med.kurume-u.ac.jp.

Abstract

Loss of mitochondrial membrane potential (ΔΨm) triggers dramatic structural changes in mitochondria from a tubular to globular shape, referred to as mitochondrial fragmentation; the resulting globular mitochondria are called swelled or ring/doughnut mitochondria. We evaluated the early period of structural changes during the ΔΨm loss-induced transformation after carbonyl cyanide m-chlorophenyl hydrazine (CCCP) administration using a newly developed correlative microscopic method combined with fluorescence microscopic live imaging and volume electron microscopy. We found that most mitochondria changed from a tubular shape to a globular shape without fusion or fission and typically showed ring shapes within 10 min after CCCP exposure. In contrast, most ring mitochondria did not have a true through hole; rather, they had various indents, and 47% showed stomatocyte shapes with vase-shaped cavities, which is the most stable physical structure without any structural support if the long tubular shape shortens into a sphere. Our results suggested that loss of ΔΨm triggered collapse of mitochondrial structural support mechanisms.

PMID:
29321618
PMCID:
PMC5762872
DOI:
10.1038/s41598-017-18582-6
[Indexed for MEDLINE]
Free PMC Article

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