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Sci Rep. 2018 Jan 10;8(1):337. doi: 10.1038/s41598-017-16535-7.

On-Chip Fluorescence Switching System for Constructing a Rewritable Random Access Data Storage Device.

Nguyen HH1,2, Park J1,2, Hwang S3, Kwon OS1,2, Lee CS1,2, Shin YB1,2, Ha TH4,5, Kim M6,7,8.

Author information

1
Hazards Monitoring Bionano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-Gu, Daejeon, 34141, Korea.
2
Department of Nanobiotechnology, Korea University of Science and Technology (UST), 217 Gajeongno, Yuseong-Gu, Daejeon, 34113, Korea.
3
Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-Gu, Daejeon, 34141, Korea.
4
Hazards Monitoring Bionano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-Gu, Daejeon, 34141, Korea. taihwan@kribb.re.kr.
5
Department of Nanobiotechnology, Korea University of Science and Technology (UST), 217 Gajeongno, Yuseong-Gu, Daejeon, 34113, Korea. taihwan@kribb.re.kr.
6
Hazards Monitoring Bionano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-Gu, Daejeon, 34141, Korea. kimm@kribb.re.kr.
7
Department of Nanobiotechnology, Korea University of Science and Technology (UST), 217 Gajeongno, Yuseong-Gu, Daejeon, 34113, Korea. kimm@kribb.re.kr.
8
Department of Pathobiology, College of Veterinary Medicine Nursing & Allied Health (CVMNAH), Tuskegee University, Tuskegee, AL, 36088, USA. kimm@kribb.re.kr.

Abstract

We report the development of on-chip fluorescence switching system based on DNA strand displacement and DNA hybridization for the construction of a rewritable and randomly accessible data storage device. In this study, the feasibility and potential effectiveness of our proposed system was evaluated with a series of wet experiments involving 40 bits (5 bytes) of data encoding a 5-charactered text (KRIBB). Also, a flexible data rewriting function was achieved by converting fluorescence signals between "ON" and "OFF" through DNA strand displacement and hybridization events. In addition, the proposed system was successfully validated on a microfluidic chip which could further facilitate the encoding and decoding process of data. To the best of our knowledge, this is the first report on the use of DNA hybridization and DNA strand displacement in the field of data storage devices. Taken together, our results demonstrated that DNA-based fluorescence switching could be applicable to construct a rewritable and randomly accessible data storage device through controllable DNA manipulations.

PMID:
29321500
PMCID:
PMC5762669
DOI:
10.1038/s41598-017-16535-7
[Indexed for MEDLINE]
Free PMC Article

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