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Biochem J. 2018 Feb 14;475(3):649-662. doi: 10.1042/BCJ20170899.

Adipose depot-specific effects of ileal interposition surgery in UCD-T2D rats: unexpected implications for obesity and diabetes.

Author information

1
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA 95616, U.S.A.
2
Department of Nutrition, University of California, Davis, CA 95616, U.S.A.
3
Department of Pharmaceutical and Pharmacological Sciences, Pharmacology Building, University of Padova, Largo Meneghetti 2, 35131 Padova, Italy.
4
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA 95616, U.S.A. cgiulivi@ucdavis.edu.
5
Medical Investigations of Neurodevelopmental Disorders (M. I. N. D.) Institute, University of California, Davis, CA 95817, U.S.A.

Abstract

Ileal interposition (IT) surgery delays the onset of diabetes in a rat model of type-2 diabetes (UCD-T2DM). Here, to gain a deeper understanding of the molecular events underlying the effects of IT surgery, we examined the changes in the proteome of four white adipose depots (retroperitoneal, mesenteric, inguinal, and epididymal) and plasma-free fatty acid profile in pre-diabetic rats 1.5 months following IT or sham surgery. The IT-mediated changes were exerted mainly in mesenteric fat and spanned from delayed adipocyte maturation to a neuroendocrine remodeling. Conversely, inguinal, retroperitoneal, and epididymal depots showed opposite trends consistent with increased adipocyte maturation and adipogenesis development prior to overt signs of diabetes, probably orchestrated by peroxisome proliferator-activated receptor gamma signaling and higher plasma n-6/n-3 free fatty acid ratios. The resulting scenario suggests a targeted use of surgical strategies that seek to delay or improve diabetes in order to manipulate adipose depot-specific responses to maximize the duration and beneficial effects of the surgery.

KEYWORDS:

lipidomics; obesity; proteomics; rat models; type 2 diabetes; white adipose tissue

PMID:
29321243
DOI:
10.1042/BCJ20170899
[Indexed for MEDLINE]
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