Send to

Choose Destination
Biofouling. 2018 Feb;34(2):132-148. doi: 10.1080/08927014.2017.1413551. Epub 2018 Jan 10.

Metabolome and proteome changes between biofilm and planktonic phenotypes of the marine bacterium Pseudoalteromonas lipolytica TC8.

Author information

a MAPIEM EA 4323 , Université de Toulon , Toulon , France.
b Fédération de Recherche FR3450 , CNRS , Toulouse , France.
c Institut de Pharmacologie et de Biologie Structurale, IPBS , Université de Toulouse, CNRS, UPS , Toulouse , France.
d Laboratoire de Recherche en Sciences Végétales , Université de Toulouse, CNRS, UPS , Castanet-Tolosan , France.


A number of bacteria adopt various lifestyles such as planktonic free-living or sessile biofilm stages. This enables their survival and development in a wide range of contrasting environments. With the aim of highlighting specific metabolic shifts between these phenotypes and to improve the overall understanding of marine bacterial adhesion, a dual metabolomics/proteomics approach was applied to planktonic and biofilm cultures of the marine bacterium Pseudoalteromonas lipolytica TC8. The liquid chromatography mass spectrometry (LC-MS) based metabolomics study indicated that membrane lipid composition was highly affected by the culture mode: phosphatidylethanolamine (PEs) derivatives were over-produced in sessile cultures while ornithine lipids (OLs) were more specifically synthesized in planktonic samples. In parallel, differences between proteomes revealed that peptidases, oxidases, transcription factors, membrane proteins and the enzymes involved in histidine biosynthesis were over-expressed in biofilms while proteins involved in heme production, nutrient assimilation, cell division and arginine/ornithine biosynthesis were specifically up-regulated in free-living cells.


Pseudoalteromonas lipolytica TC8; biofilms; liquid chromatography-mass spectrometry; membrane lipids; metabolomics; proteomics

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center