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Biosci Biotechnol Biochem. 2018 Feb;82(2):258-267. doi: 10.1080/09168451.2017.1415127. Epub 2018 Jan 10.

Cloning of a novel lectin from Artocarpus lingnanensis that induces apoptosis in human B-lymphoma cells.

Author information

1
a Department of Biochemistry and Molecular Biology , Guangxi Medical University , Guangxi , P.R. China.
2
b Clinical Laboratory , First Affiliated Hospital of Guangxi Medical University , Guangxi , P. R. China.
3
c Department of Orthopaedics , Orthopaedics Hospital , Guangxi , P.R. China.
4
d Key Laboratory of Biological Molecular Medicine Research , Guangxi Medical University , Guangxi , P. R. China.

Abstract

We isolated a novel lectin (Artocarpus nitidus subsp. lingnanensis lectin, ALL) from Artocarpus nitidus subsp. lingnanensis and showed its mitogenic activities. In this study, we determined the amino acid sequence of ALL by cDNA sequencing. ALL cDNA (933 bp) contains a 657-bp open reading frame (ORF), which encodes a protein with 218 amino acids. ALL shares high sequence similarities with Jacalin and Morniga G and belongs to jacalin-related lectin family. We also examined the antitumor activity of ALL using Raji, a human B-lymphoma cell line. ALL exhibits a strong binding affinity to cell membrane, which can be effectively inhibited by N-acetyl-D-galactosamine (GalNAc). ALL inhibits Raji cell proliferation in a time- and dose-dependent manner through apoptosis, evidenced by morphological changes, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Bcl-2 down-regulation, and caspase-3 activation. We further showed that the activation of p38 mitogen-activated protein kinase (MAPK) signaling pathways is required for the pro-apoptotic activity of ALL.

KEYWORDS:

ALL: Artocarpus nitidus subsp. lingnanensis lectin; DAPI: 4′,6-diamidino-2-phenylindole; ERK: extracellular signal-regulated kinase; FITC: fluorescein isothiocyanate; GalNAc: N-acetyl-D-galactosamine; JNK: c-jun N-terminal kinase; JRLs: jacalin-related lectins; Lectin; MAPK: mitogen-activated protein kinase; MFI: median fluorescence intensity; MPA: Maclura pomifera lectin; ORF: open reading frame; PARP: poly ADP-ribose polymerase; PCR: polymerase chain reaction; RACE: rapid-amplification of cDNA ends; antitumor drug; apoptosis; cDNA cloning

PMID:
29318910
DOI:
10.1080/09168451.2017.1415127
[Indexed for MEDLINE]

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