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Chem Biol Interact. 2018 Feb 25;282:63-68. doi: 10.1016/j.cbi.2018.01.005. Epub 2018 Jan 6.

Arctigenin protects against ultraviolet-A-induced damage to stemness through inhibition of the NF-κB/MAPK pathway.

Author information

1
Department of Bio and Chemical Engineering, Hongik University, 300-16 Sejong City, Republic of Korea.
2
Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon City, 164-19 Gyunggi Do, Republic of Korea.
3
Skincure Life Science Institute, Seongnam City, 132-16 Gyunggi Do, Republic of Korea.
4
Viral Disease Research Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si, 39660 Gyeongsangbuk-do, Republic of Korea.
5
Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, 131-20 Gyunggi Do, Republic of Korea. Electronic address: leesaye@gachon.ac.kr.
6
Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon City, 164-19 Gyunggi Do, Republic of Korea. Electronic address: bioneer@skku.edu.

Abstract

The stemness of stem cells is negatively affected by ultraviolet A (UVA) irradiation. This study was performed to examine the effects of arctigenin on UVA-irradiation-induced damage to the stemness of human mesenchymal stem cells (hMSCs) derived from adipose tissue. The mechanisms of action of arctigenin were also investigated. A BrdU-incorporation assay demonstrated that arctigenin attenuated the UVA-induced reduction of the cellular proliferative potential. Arctigenin also increased the UVA-induced reduction in stemness of hMSCs by upregulating stemness-related genes such as SOX2, OCT4, and NANOG. In addition, the UVA-induced reduction in the mRNA expression level of hypoxia-inducible factor (HIF)-1α was significantly recovered by arctigenin. The antagonizing effect of arctigenin on UVA irradiation was mediated by reduced PGE2 production through the inhibition of MAPKs (p42/44 MAPK, p38 MAPK, and JNK) and NF-κB. Overall, these findings suggest that arctigenin can ameliorate the reduced stemness of hMSCs induced by UVA irradiation. The effects of arctigenin are mediated by PGE2-cAMP signaling-dependent upregulation of HIF-1α. Therefore, arctigenin could be used as an antagonist to attenuate the effects of UVA irradiation.

KEYWORDS:

Arctigenin; HIF; Human mesenchymal stem cells; Stemness; UVA damage

PMID:
29317250
DOI:
10.1016/j.cbi.2018.01.005
[Indexed for MEDLINE]

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