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J Cell Biochem. 2018 May;119(5):4216-4223. doi: 10.1002/jcb.26661. Epub 2018 Jan 25.

Testican-1, as a novel diagnosis of sepsis.

Author information

1
College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, BK21 Plus KNU Multi-Omics based Creative Drug Research Team, Kyungpook National University, Daegu, Republic of Korea.
2
Aging Research Center, Korea Research Institute of Bioscience and Biotechnology, Deajeon, Republic of Korea.
3
Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
4
Department of Biochemistry, School of Medicine, Gachon University, Incheon, Republic of Korea.

Abstract

The development of new sepsis-specific biomarkers is mandatory to improve the detection and monitoring of the disease. Testican-1 is a highly conserved, multidomain proteoglycan that is most prominently expressed in the thalamus of the brain, and is upregulated in activated astroglial cells of the cerebrum. The aim of this study was to evaluate blood levels of Testican-1 in septic patients. A prospective study of 82 patients with sepsis was conducted. Furthermore, C57BL/6 mice were administrated with lipopolysaccharide (LPS), high mobility group box 1 (HMGB1) protein or subjected to cecal ligation and puncture (CLP) surgery. Alternatively, human umbilical vein endothelial cells (HUVECs) or C57BL/6 mice were exposed to LPS (100 ng/mL) or HMGB1 (1 μg/mL). LPS, HMGB1, or CLP enhanced the synthesis and secretion of Testican-1 in HUVECs and mice. In patients admitted to the intensive care unit (ICU) with sepsis, circulating levels of Testican-1 were significantly high (sepsis, 20.44-63.37 ng/mL, n = 30; severe sepsis, 41.30-98.69 ng/mL, n = 22; septic shock, 98.10-151.85 ng/mL, n = 30) when compared to the levels of control donors (6.97-8.77 ng/mL, n = 21), reflecting the severity of the disease. These results suggest that in septic patients, Testican-1 blood level is related to the severity of sepsis and Testican-1 could be used as a biomarker to determine the severity of sepsis.

KEYWORDS:

CLP; Testican-1; inflammation; marker; sepsis

PMID:
29315764
DOI:
10.1002/jcb.26661
[Indexed for MEDLINE]

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