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Monoclon Antib Immunodiagn Immunother. 2018 Feb;37(1):59-62. doi: 10.1089/mab.2017.0052. Epub 2018 Jan 9.

Development of an Anti-HER2 Monoclonal Antibody H2Mab-139 Against Colon Cancer.

Author information

1
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Japan .
2
2 New Industry Creation Hatchery Center, Tohoku University , Sendai, Japan .

Abstract

Human epidermal growth factor receptor 2 (HER2) expression has been reported in several cancers, such as breast, gastric, lung, pancreatic, and colorectal cancers. HER2 is overexpressed in those cancers and is associated with poor clinical outcomes. Trastuzumab, a humanized anti-HER2 antibody, provides significant survival benefits for patients with HER2-overexpressing breast cancers and gastric cancers. In this study, we developed a novel anti-HER2 monoclonal antibody (mAb), H2Mab-139 (IgG1, kappa) and investigated it against colon cancers using flow cytometry, western blot, and immunohistochemical analyses. Flow cytometry analysis revealed that H2Mab-139 reacted with colon cancer cell lines, such as Caco-2, HCT-116, HCT-15, HT-29, LS 174T, COLO 201, COLO 205, HCT-8, SW1116, and DLD-1. Although H2Mab-139 strongly reacted with LN229/HER2 cells on the western blot, we did not observe a specific signal for HER2 in colon cancer cell lines. Immunohistochemical analyses revealed sensitive and specific reactions of H2Mab-139 against colon cancers, indicating that H2Mab-139 is useful in detecting HER2 overexpression in colon cancers using flow cytometry and immunohistochemical analyses.

KEYWORDS:

HER2; colon cancer; immunohistochemistry; monoclonal antibody

PMID:
29315012
DOI:
10.1089/mab.2017.0052
[Indexed for MEDLINE]

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