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Inflammopharmacology. 2018 Oct;26(5):1233-1243. doi: 10.1007/s10787-017-0433-1. Epub 2018 Jan 8.

Polar extract of Curcuma longa protects cartilage homeostasis: possible mechanism of action.

Author information

1
Department of Biology, R&D Centre, Natural Remedies Private Limited, 5B, Veerasandra Indl. Area, 19th K. M. Stone, Hosur Road, Electronic City Post, Bangalore, Karnataka, 560100, India. cvc@naturalremedy.com.
2
Department of Biology, R&D Centre, Natural Remedies Private Limited, 5B, Veerasandra Indl. Area, 19th K. M. Stone, Hosur Road, Electronic City Post, Bangalore, Karnataka, 560100, India.

Abstract

BACKGROUND:

Curcuma longa has been well documented for managing joint inflammation and pain. The present study investigated the effect of polar extract of C. longa (NR-INF-02) on cartilage homeostasis in human articular chondrocytes knee (NHAC-kn) cells to understand its plausible mechanism of action.

METHODS:

Dysregulation of cartilage homeostasis was induced by IL-1β and H2O2. Modulating effects of NR-INF-02 on degradation markers viz., chondrocyte apoptosis, senescence, cytokine, eicosanoids, and cartilage synthesis markers viz., glycosaminoglycans and type II collagen degradation was evaluated in human articular chondrocytes knee (NHAC-kn) cells. Further, the effect of NR-INF-02 on lipopolysaccharide (LPS)-induced expression of NF-kB in RAW264.7 macrophages was investigated.

RESULTS:

NR-INF-02 significantly attenuated IL-1β-induced chondrocyte cytotoxicity, apoptosis and release of chondrocyte degradation markers such as IL-6, IL-8, COX-2, PGE2, TNF-α, ICAM-1 in NHAC-kn cells. Also, NR-INF-02 protected IL-1β-induced damage to synthesis markers such as glycosaminoglycans, type II collagen and further attenuated H2O2-induced chondrocyte senescence. In addition NR-INF-02 suppressed LPS-induced NF-kB expression in RAW264.7 cells.

CONCLUSIONS:

NR-INF-02 protects cartilage homeostasis by maintaining the balance between synthesis and degradation of cartilage matrix.

KEYWORDS:

Cartilage homeostasis; Curcuma longa; NR-INF-02; Osteoarthritis; Pain; Senescence; Turmacin

PMID:
29313174
DOI:
10.1007/s10787-017-0433-1
[Indexed for MEDLINE]

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