Format

Send to

Choose Destination
Front Mol Neurosci. 2017 Dec 19;10:420. doi: 10.3389/fnmol.2017.00420. eCollection 2017.

Silencing NUDT21 Attenuates the Mesenchymal Identity of Glioblastoma Cells via the NF-κB Pathway.

Author information

1
Department of Neurosurgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
2
Department of Anesthesiology, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
3
Health Management Center, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
4
Department of Neurology, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.

Abstract

The proneural (PN) and mesenchymal (MES) subtypes of glioblastoma multiforme (GBM) are robust and generally consistent with classification schemes. GBMs in the MES subclass are predominantly primary tumors that, compared to PN tumors, exhibit a worse prognosis; thus, understanding the mechanism of MES differentiation may be of great benefit for the treatment of GBM. Nuclear factor kappa B (NF-κB) signaling is critically important in GBM, and activation of NF-κB could induce MES transdifferentiation in GBM, which warrants additional research. NUDT21 is a newly discovered tumor-associated gene according to our current research. The exact roles of NUDT21 in cancer incidence have not been elucidated. Here, we report that NUDT21 expression was upregulated in human glioma tissues and that NUDT21 promoted glioma cell proliferation, likely through the NF-κB signaling pathway. Gene set enrichment analysis, western blotting, and quantitative real-time reverse transcription polymerase chain reaction confirmed that NF-κB inhibitor zeta (NFKBIZ) was a downstream target affected by NUDT21 and that the MES identity genes in glioblastoma cells, CHI3L1 and FN1, were also differentially regulated. Our results suggest that NUDT21 is an upstream regulator of the NF-κB pathway and a potential molecular target for the MES subtype of GBM.

KEYWORDS:

NUDT21; glioblastoma; mesenchymal identity; microarray; therapy

Supplemental Content

Full text links

Icon for Frontiers Media SA Icon for PubMed Central
Loading ...
Support Center