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Nat Commun. 2018 Jan 8;9(1):82. doi: 10.1038/s41467-017-02642-6.

Interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes.

Author information

1
Neuroscience Institute, National Research Council (CNR), 35121, Padova, Italy.
2
Department of Biomedical Sciences, Università degli Studi di Padova, 35121, Padova, Italy.
3
Department of Experimental and Clinical Medicine, Università Politecnica delle Marche, 60126, Ancona, Italy.
4
Center for Neurobiology of Aging, INRCA IRCCS, 60121, Ancona, Italy.
5
Optical Approches to Brain Function Laboratory, Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 16163, Genova, Italy.
6
Inserm UMR894, Center for Psychiatry and Neuroscience, Université Paris-Descartes, 75014, Paris, France.
7
Departement of Biomedicine, University of Basel, 4031, Basel, Switzerland.
8
Foundation for Molecular Medicine, Università Politecnica delle Marche, 60126, Ancona, Italy.
9
Neuroscience Institute, National Research Council (CNR), 35121, Padova, Italy. giorgio.carmignoto@bio.unipd.it.
10
Department of Biomedical Sciences, Università degli Studi di Padova, 35121, Padova, Italy. giorgio.carmignoto@bio.unipd.it.

Abstract

The signaling diversity of GABAergic interneurons to post-synaptic neurons is crucial to generate the functional heterogeneity that characterizes brain circuits. Whether this diversity applies to other brain cells, such as the glial cells astrocytes, remains unexplored. Using optogenetics and two-photon functional imaging in the adult mouse neocortex, we here reveal that parvalbumin- and somatostatin-expressing interneurons, two key interneuron classes in the brain, differentially signal to astrocytes inducing weak and robust GABAB receptor-mediated Ca2+ elevations, respectively. Furthermore, the astrocyte response depresses upon parvalbumin interneuron repetitive stimulations and potentiates upon somatostatin interneuron repetitive stimulations, revealing a distinguished astrocyte plasticity. Remarkably, the potentiated response crucially depends on the neuropeptide somatostatin, released by somatostatin interneurons, which activates somatostatin receptors at astrocytic processes. Our study unveils, in the living brain, a hitherto unidentified signaling specificity between interneuron subtypes and astrocytes opening a new perspective into the role of astrocytes as non-neuronal components of inhibitory circuits.

PMID:
29311610
PMCID:
PMC5758790
DOI:
10.1038/s41467-017-02642-6
[Indexed for MEDLINE]
Free PMC Article

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