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J Microbiol Methods. 2018 Feb;145:69-72. doi: 10.1016/j.mimet.2017.12.013. Epub 2018 Jan 5.

New colony multiplex PCR assays for the detection and discrimination of vancomycin-resistant enterococcal species.

Author information

1
Department of Bacteriology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
2
Laboratory of Bacterial Drug Resistance, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
3
Institute of Clinical Pharmacology, Peking University First Hospital, Number 8, Xishiku Street, Xicheng District, Beijing 100034, China.
4
Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
5
Department of Bacteriology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan; Laboratory of Bacterial Drug Resistance, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan. Electronic address: tomitaha@gunma-u.ac.jp.

Abstract

New colony multiplex PCR assays for detection of seven types of vancomycin-resistance determinants and eight types of Enterococcus species were developed. For 135 enterococcal isolates examined in this study, these assays showed high sensitivity and specificity, and could provide the rapid and accurate detection of vancomycin-resistant determinants and Enterococcus spp.

KEYWORDS:

Colony multiplex PCR; Drug resistance; Enterococcus spp.; Vancomycin-resistant enterococci

PMID:
29309802
DOI:
10.1016/j.mimet.2017.12.013
[Indexed for MEDLINE]

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