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World J Gastroenterol. 2017 Dec 21;23(47):8345-8354. doi: 10.3748/wjg.v23.i47.8345.

Circular RNA circ-LDLRAD3 as a biomarker in diagnosis of pancreatic cancer.

Author information

1
Endoscopy Center, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.
2
Medical Research Center, Shengjing Hospital of China Medical University, Benxi 117000, Liaoning Province, China.
3
Endoscopy Center, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China. sun-siyu@163.com.

Abstract

AIM:

To analyze the diagnostic value of a circular RNA (circRNA), circ-LDLRAD3, in pancreatic cancer.

METHODS:

Expression levels of circ-LDLRAD3 were tested in both cells and clinical samples; the latter included 30 paired pancreatic cancer tissues and adjacent non-tumorous tissues, 31 plasma samples from patients with pancreatic cancer, and 31 plasma samples from healthy volunteers. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to measure expression levels of circ-LDLRAD3 in cells and clinical samples; then, the relationship between clinicopathological factors of patient samples and expression of circ-LDLRAD3 in pancreatic cancer was analyzed. The diagnostic value of circ-LDLRAD3 was verified by receiver operating characteristic (ROC) curve analysis.

RESULTS:

Circ-LDLRAD3 was up-regulated in pancreatic cancer cell lines (P < 0.01), pancreatic cancer tissues (P < 0.01), and plasma samples from patients with pancreatic cancer (P < 0.01). High expression of circ-LDLRAD3 was significantly associated with venous invasion, lymphatic invasion, and metastasis. The area under the ROC curve of circ-LDLRAD3 alone or combination with CA19-9 was 0.67 and 0.87, respectively, with a sensitivity and specificity of 0.5738 (alone) and 0.7049 (alone), and 0.8033 (combination) and 0.9355 (combination), respectively.

CONCLUSION:

These data suggest that circ-LDLRAD3 may be a biomarker in the diagnosis of pancreatic cancer.

KEYWORDS:

Biomarker; Circular RNA; Pancreatic cancer

PMID:
29307994
PMCID:
PMC5743505
DOI:
10.3748/wjg.v23.i47.8345
[Indexed for MEDLINE]
Free PMC Article

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