Format

Send to

Choose Destination
Mol Genet Metab. 2018 Mar;123(3):400-410. doi: 10.1016/j.ymgme.2017.11.010. Epub 2017 Nov 28.

Endurance training reduces exercise-induced acidosis and improves muscle function in a mouse model of sickle cell disease.

Author information

1
Aix-Marseille Univ, CNRS, CRMBM, Marseille, France. Electronic address: benjamin.chatel@live.fr.
2
Aix-Marseille Univ, CNRS, CRMBM, Marseille, France; Université Savoie Mont Blanc, Laboratoire Interuniversitaire de Biologie de la Motricité, EA 7424, F-73000 Chambéry, France.
3
Univ Lyon, Université Claude Bernard Lyon 1, Laboratoire Interuniversitaire de Biologie de la Motricité, EA 7424, Villeurbanne, France.
4
Aix-Marseille Univ, CNRS, CRMBM, Marseille, France.
5
Paris Descartes University, Institute for Research in bioMedicine and Epidemiology of Sport, Paris, France.

Abstract

Sickle cell disease (SCD) mice (Townes model of SCD) presented exacerbated exercise-induced acidosis and fatigability as compared to control animals. We hypothesize that endurance training could represent a valuable approach to reverse these muscle defects. Endurance-trained HbAA (HbAA-END, n=10), HbAS (HbAS-END, n=11) and HbSS (HbSS-END, n=8) mice were compared to their sedentary counterparts (10 HbAA-SED, 10 HbAS-SED and 9 HbSS-SED mice) during two rest - exercise - recovery protocols during which muscle energetics and function were measured. In vitro analyses of some proteins involved in muscle energetics, pH regulation and oxidative stress were also performed. Exercise-induced acidosis was lower in HbSS-END mice as compared to their sedentary counterparts during both moderate (p<0.001) and intense (p<0.1) protocols. The total force production measured during both protocols was higher in trained mice compared to sedentary animals. In vitro analyses revealed that enolase/citrate synthase ratio was reduced in HbSS-END (p<0.001) and HbAS-END (p<0.01) mice compared to their sedentary counterparts. In addition, malondialdehyde concentration was reduced in trained mice (p<0.05). In conclusion, endurance training would reverse the more pronounced exercise-induced acidosis, reduce oxidative stress and ameliorate some of the muscle function parameters in SCD mice.

KEYWORDS:

Muscle energetics; Muscle force production; Physical activity; pH

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center