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Cell. 2018 Jan 11;172(1-2):205-217.e12. doi: 10.1016/j.cell.2017.12.007. Epub 2018 Jan 4.

Multiclonal Invasion in Breast Tumors Identified by Topographic Single Cell Sequencing.

Author information

1
Department of Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA; Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
2
Department of Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
3
Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
4
Department of Investigational Cancer Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
5
Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. Electronic address: medgerton@mdanderson.org.
6
Department of Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA; Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center, Houston, TX, USA; Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. Electronic address: nnavin@mdanderson.org.

Abstract

Ductal carcinoma in situ (DCIS) is an early-stage breast cancer that infrequently progresses to invasive ductal carcinoma (IDC). Genomic evolution has been difficult to delineate during invasion due to intratumor heterogeneity and the low number of tumor cells in the ducts. To overcome these challenges, we developed Topographic Single Cell Sequencing (TSCS) to measure genomic copy number profiles of single tumor cells while preserving their spatial context in tissue sections. We applied TSCS to 1,293 single cells from 10 synchronous patients with both DCIS and IDC regions in addition to exome sequencing. Our data reveal a direct genomic lineage between in situ and invasive tumor subpopulations and further show that most mutations and copy number aberrations evolved within the ducts prior to invasion. These results support a multiclonal invasion model, in which one or more clones escape the ducts and migrate into the adjacent tissues to establish the invasive carcinomas.

KEYWORDS:

breast cancer; breast cancer progression; cancer genomics; clonal evolution; copy number evolution; ductal carcinoma in situ; genome evolution; intratumor heterogeneity; invasion; single-cell sequencing

PMID:
29307488
PMCID:
PMC5766405
DOI:
10.1016/j.cell.2017.12.007
[Indexed for MEDLINE]
Free PMC Article

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