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Sci China Life Sci. 2018 May;61(5):541-549. doi: 10.1007/s11427-017-9138-9. Epub 2018 Jan 2.

miR-137 inhibits melanoma cell proliferation through downregulation of GLO1.

Author information

1
Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Universities of the Confederated Institute for Proteomics, Beijing Normal University, Beijing, 100875, China.
2
Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing, 100048, China.
3
Gene & Care Health Technologies, Co., Ltd., Shanghai, 201203, China.
4
GMP & T Cell Therapy Unit, German Cancer Research Center (DKFZ), Heidelberg, D-69120, Germany.
5
College of Sports, Northwest Normal University, Lanzhou, 730070, China.
6
Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Universities of the Confederated Institute for Proteomics, Beijing Normal University, Beijing, 100875, China. dhe@bnu.edu.cn.

Abstract

Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The glyoxalase system member glyoxalase 1 (GLO1) is the principal scavenging enzyme of methylglyoxal (MG), a toxic byproduct of glycolysis. Using 35S in vivo/vitro labelling analysis for dynamic proteomics (SiLAD), we found that miR-137 downregulated the expression of GLO1 in melanoma cells. Bioinformatics analysis predicted that GLO1 is a direct target of miR-137. This was validated by dual luciferase reporter assay. Quantitative RT-PCR (qRT-PCR) and western blot analysis indicated that miR-137 could decrease endogenous GLO1 expression. Furthermore, siRNA targeting of GLO1 mimicked inhibition of melanoma cell proliferation caused by miR-137 overexpression. Re-expression of GLO1 was able to restore miR-137-mediated suppression of melanoma cell proliferation. Therefore, these results suggest that miR-137 inhibits the proliferation of melanoma cells by targeting GLO1.

KEYWORDS:

GLO1; SiLAD; cell proliferation; melanoma; miR-137

PMID:
29307109
DOI:
10.1007/s11427-017-9138-9

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