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PLoS One. 2018 Jan 5;13(1):e0190573. doi: 10.1371/journal.pone.0190573. eCollection 2018.

Positive associations between upregulated levels of stress-induced phosphoprotein 1 and matrix metalloproteinase-9 in endometriosis/adenomyosis.

Author information

1
Department of Obstetrics and Gynecology, LinKou Medical Center, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan.
2
Genomic Medicine Research Core Laboratory, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
3
Department of Laboratory Medicine, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
4
Gynecologic Cancer Research Centre, LinKou Medical Center, Chang Gung Memorial Hospital, Taoyuan Taiwan.
5
Department of Clinical Pathology, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
6
Graduate Institutes of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
7
Department of Biotechnology, Ming-Chuan University, Taoyuan, Taiwan.

Abstract

Stress-induced phosphoprotein-1 (STIP1), an adaptor protein that coordinates the functions of HSP70 and HSP90 in protein folding, has been implicated in the development of human gynecologic malignancies. This case-control study investigates STIP1 serum levels and tissue expression in relation to endometriosis/adenomyosis in Taiwanese population. Female patients with surgically confirmed endometriosis/adenomyosis were compared with women free of endometriosis/adenomyosis. Serum STIP1 levels were measured using an enzyme-linked immunosorbent assay and surgical tissues were analyzed by immunohistochemistry. Both epithelial and stromal cells in surgical tissues of endometriosis and adenomyosis expressed STIP1 and MMP-9. Notably, MMP-9 expression was significantly decreased when STIP1 expression was knocked-down. In vitro experiments revealed that STIP1 was capable of binding to the MMP-9 promoter and enhanced its transcriptional expression. The preoperative serum STIP1 levels of patients with endometriosis/adenomyosis were significantly higher than those of the controls. In brief, our data suggest an association between STIP1 levels and endometriosis/adenomyosis.

PMID:
29304094
PMCID:
PMC5755831
DOI:
10.1371/journal.pone.0190573
[Indexed for MEDLINE]
Free PMC Article

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