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Genetics. 2018 Jan;208(1):1-18. doi: 10.1534/genetics.117.1113.

Advances in Engineering the Fly Genome with the CRISPR-Cas System.

Author information

1
Cell and Developmental Biology, University of California, San Diego, La Jolla, California 92093-0349 ebier@ucsd.edu mharrison3@wisc.edu oconnorgiles@wisc.edu wildonger@wisc.edu.
2
Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706 ebier@ucsd.edu mharrison3@wisc.edu oconnorgiles@wisc.edu wildonger@wisc.edu.
3
Laboratory of Genetics and Laboratory of Cell and Molecular Biology, Wisconsin 53706 ebier@ucsd.edu mharrison3@wisc.edu oconnorgiles@wisc.edu wildonger@wisc.edu.
4
Biochemistry Department, University of Wisconsin-Madison, Wisconsin 53706 ebier@ucsd.edu mharrison3@wisc.edu oconnorgiles@wisc.edu wildonger@wisc.edu.

Abstract

Drosophila has long been a premier model for the development and application of cutting-edge genetic approaches. The CRISPR-Cas system now adds the ability to manipulate the genome with ease and precision, providing a rich toolbox to interrogate relationships between genotype and phenotype, to delineate and visualize how the genome is organized, to illuminate and manipulate RNA, and to pioneer new gene drive technologies. Myriad transformative approaches have already originated from the CRISPR-Cas system, which will likely continue to spark the creation of tools with diverse applications. Here, we provide an overview of how CRISPR-Cas gene editing has revolutionized genetic analysis in Drosophila and highlight key areas for future advances.

KEYWORDS:

CRISPR-Cas; Drosophila; FlyBook; gene drive; gene editing; genome engineering

PMID:
29301946
PMCID:
PMC5753851
DOI:
10.1534/genetics.117.1113
[Indexed for MEDLINE]
Free PMC Article

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