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Materials (Basel). 2018 Jan 1;11(1). pii: E61. doi: 10.3390/ma11010061.

Enhancing of Osseointegration with Propolis-Loaded TiO₂ Nanotubes in Rat Mandible for Dental Implants.

Author information

1
Department of Dental Biomaterials, Institute of Biodegradable Materials, BK21 plus Program, School of Dentistry, Chonbuk National University, JeonJu 54896, Korea. nithideth@hotmail.com.
2
Department of Prosthodontics, University of Health Sciences, Vientiane 7444, Laos. nithideth@hotmail.com.
3
Department of Dental Biomaterials, Institute of Biodegradable Materials, BK21 plus Program, School of Dentistry, Chonbuk National University, JeonJu 54896, Korea. yk0830@naver.com.
4
Department of Dental Biomaterials, Institute of Biodegradable Materials, BK21 plus Program, School of Dentistry, Chonbuk National University, JeonJu 54896, Korea. pooh3180@hotmail.com.
5
Department of Molecular Biology and the Institute for Molecular biology and Gemetics, Chonbuk National University, JeonJu 54896, Korea. leeyh@jbnu.ac.kr.
6
Department of Oral Biochemistry, Institute of Oral Bioscience, BK21 plus Program, School of Dentistry, Chonbuk National University, JeonJu 54896, Korea. yihokn@chonbuk.ac.kr.
7
Department of Dental Hygiene, Hanseo University, Seosan 31962, Korea. jhjang@hanseo.ac.kr.
8
Department of Oral and Maxillofacial Radiology, School of Dentistry and Institute of Oral Bio Science, Chonbuk National University, JeonJu 54896, Korea. beam@jbnu.ac.kr.
9
Department of Dental Biomaterials, Institute of Biodegradable Materials, BK21 plus Program, School of Dentistry, Chonbuk National University, JeonJu 54896, Korea. bts@jbnu.ac.kr.
10
Department of Dental Biomaterials, Institute of Biodegradable Materials, BK21 plus Program, School of Dentistry, Chonbuk National University, JeonJu 54896, Korea. mh@jbnu.ac.kr.

Abstract

TiO₂ nanotubes (TNT) formation is beneficial for improving bone cell-material interaction and drug delivery for Ti dental implants. Among the natural drugs to be installed in TNT, selected propolis has antibacterial and anti-inflammatory properties. It is a resinous natural product which is collected by the honeybees from the various types of plants with their salivary enzymes. This study concludes that TNT loaded with a propolis (PL-TNT-Ti) dental implant has the ability to improve osseointegration. The propolis particles were embedded within the TNT or adhered to the top. In a cytotoxicity test using osteoblast, PL-TNT-Ti group exhibited an increased cell proliferation and differentiation. A Sprague Dawley rat mandibular model was used to evaluate the osseointegration and bone bonding of TNT or PL-TNT-Ti. From the µ-CT and hematoxylin and eosin (HE) histological results after implantation at 1 and 4 weeks to rat mandibular, an increase in the extent of new bone formation and mineral density around the PL-TNT-Ti implant was confirmed. The Masson's trichrome staining showed the expression of well-formed collagenous for bone formation on the PL-TNT-Ti. Immunohistochemistry staining indicate that bone morphogenetic proteins (BMP-2 and BMP-7) around the PL-TNT-Ti increased the expression of collagen fibers and of osteogenic differentiation whereas the expression of inflammatory cytokine such as interleukin-1 beta (IL-1ß) and tumor necrosis factor-alpha (TNF-α) is decreased.

KEYWORDS:

dental implant; mandibular injuries; nanotubes; osseointegration; propolis

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