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Immunopharmacol Immunotoxicol. 2018 Feb;40(1):83-90. doi: 10.1080/08923973.2017.1414836. Epub 2018 Jan 4.

Anti-inflammatory effects of embelin in A549 cells and human asthmatic airway epithelial tissues.

Lee IS1,2, Cho DH2,3, Kim KS1, Kim KH1,2, Park J1,2, Kim Y1,2, Jung JH1, Kim K2,3, Jung HJ2,3, Jang HJ1,2,3.

Author information

1
a College of Korean Medicine , Kyung Hee University , Seoul , Republic of Korea.
2
b Department of Science in Korean Medicine , Graduate School, Kyung Hee University , Seoul , Republic of Korea.
3
c Department of Biological Sciences in Korean Medicine , Graduate School, Kyung Hee University , Seoul , Republic of Korea.

Abstract

OBJECTIVE:

Allergic asthma is the most common type in asthma, which is defined as a chronic inflammatory disease of the lung. In this study, we investigated whether embelin (Emb), the major component of Ardisia japonica BL. (AJB), exhibits anti-inflammatory effects on allergic asthma via inhibition of NF-κB activity using A549 cells and asthmatic airway epithelial tissues.

METHODS:

Inflammation was induced in A549 cells, a human airway epithelial cell line, by IL-1β (10 ng/ml) treatment for 4 h. The effects of Emb on NF-κB activity and COX-2 protein expression in inflamed airway epithelial cells and human asthmatic airway epithelial tissues were analyzed via western blot. The secretion levels of NF-κB-mediated cytokines/chemokines, including IL-4, 6, 9, 13, TNF-α and eotaxin, were measured by a multiplex assay.

RESULTS:

Emb significantly blocked NF-κB activity in IL-1β-treated A549 cells and human asthmatic airway epithelial tissues. COX-2 expression was also reduced in both IL-1β-treated A549 cells and asthmatic tissues Emb application. Emb significantly reduced the secretion of IL-4, IL-6 and eotaxin in human asthmatic airway epithelial tissues by inhibiting activity of NF-κB.

CONCLUSIONS:

The results of this study suggest that Emb may be used as an anti-inflammatory agent via inhibition of NF-κB and related cytokines.

KEYWORDS:

COX-2; Embelin; allergic asthma; human asthmatic airway epithelial tissue; nuclear factor kappa B

PMID:
29299941
DOI:
10.1080/08923973.2017.1414836
[Indexed for MEDLINE]

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