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J Microbiol. 2018 Jan;56(1):49-55. doi: 10.1007/s12275-018-7319-9. Epub 2018 Jan 4.

Diversity of fungi associated with roots of Calanthe orchid species in Korea.

Author information

1
School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, 08826, Republic of Korea.
2
University College, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
3
Department of Biology, Deajeon University, Daejeon, 34520, Republic of Korea.
4
National Institute of Biological Resources, Incheon, 22689, Republic of Korea.
5
School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, 08826, Republic of Korea. ywlim@snu.ac.kr.

Abstract

While symbiotic fungi play a key role in the growth of endangered Calanthe orchid species, the relationship between fungal diversity and Calanthe species remains unclear. Here, we surveyed root associated fungal diversity of six Calanthe orchid species by sequencing the internal transcribed spacer (ITS) region using 454 pyrosequencing. Our results revealed that Paraboeremia and Coprinopsis are dominant fungal genera among Calanthe species. In terms of overall relative abundance, Paraboeremia was the most common fungal genus associated with Calanthe roots, followed by Coprinopsis. Overall fungal diversity showed a significant degree of variation depending on both location and Calanthe species. In terms of number of different fungal genera detected within Calanthe species, C. discolor had the most diverse fungal community, with 10 fungal genera detected. This study will contribute toward a better understanding of those fungi that are required for successful cultivation and conservation of Korean Calanthe species.

KEYWORDS:

Calanthe; fungal diversity; next-generation sequencing; orchid-related fungi; orchidaceae

PMID:
29299843
DOI:
10.1007/s12275-018-7319-9
[Indexed for MEDLINE]

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