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Plant Cell. 2018 Jan;30(1):153-166. doi: 10.1105/tpc.17.00671. Epub 2018 Jan 3.

Histone Deacetylases SRT1 and SRT2 Interact with ENAP1 to Mediate Ethylene-Induced Transcriptional Repression.

Zhang F1,2, Wang L1,2, Ko EE1, Shao K1, Qiao H3,2.

Author information

1
Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712.
2
Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712.
3
Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712 hqiao@austin.utexas.edu.

Abstract

Ethylene plays pleiotropic roles in plant growth, plant development, and stress responses. Although the effects of ethylene on plants are well documented, little is known about molecular-level events that result in transcriptional repression during the ethylene response. In this study, we found that two histone deacetylases, SRT1 and SRT2, interact with ENAP1, which associates with EIN2 in the nucleus. Genetic and transcriptome analyses revealed that SRT1 and SRT2 are required for negative regulation of certain ethylene-responsive genes. The acetylation of HISTONE3 at K9 (H3K9Ac) is specifically regulated by SRT1 and SRT2 in ethylene-repressed genes. In addition, the srt1 srt2 double mutation in Arabidopsis thaliana suppresses both the ENAP1ox and the EIN3ox constitutive ethylene response phenotypes, and the ethylene-induced transcriptional repression observed in EIN3ox plants is derepressed in the EIN3ox/srt1 srt2 mutant. SRT2 and ENAP1 both bind to promoter regions of genes negatively regulated by ethylene, reducing H3K9Ac levels and resulting in transcriptional repression. This work establishes a mechanism by which histone deacetylases SRT1 and SRT2 interact with ENAP1 to mediate transcriptional repression by regulating the levels of H3K9 acetylation in the ethylene signaling.

PMID:
29298835
PMCID:
PMC5810571
DOI:
10.1105/tpc.17.00671
[Indexed for MEDLINE]
Free PMC Article

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