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New Phytol. 2018 Apr;218(1):232-241. doi: 10.1111/nph.14957. Epub 2018 Jan 2.

The BIG protein distinguishes the process of CO2 -induced stomatal closure from the inhibition of stomatal opening by CO2.

Author information

1
State Key Laboratory of Hybrid Rice, Department of Plant Sciences, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
2
School of Biological Sciences, Life Sciences Building, 24 Tyndall Avenue, Bristol, BS8 1TQ, UK.
3
Lancaster Environment Centre, Lancaster University, Lancaster, LA1 4YQ, UK.
4
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
5
Cell and Developmental Biology Section, Division of Biological Sciences, University of California at San Diego, La Jolla, CA, 92093, USA.
6
School of Environmental Sciences, University of Hull, Hull, HU6 7RX, UK.
7
Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Schlossplatz 7, Münster, 48149, Germany.
8
Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK.

Abstract

We conducted an infrared thermal imaging-based genetic screen to identify Arabidopsis mutants displaying aberrant stomatal behavior in response to elevated concentrations of CO2 . This approach resulted in the isolation of a novel allele of the Arabidopsis BIG locus (At3g02260) that we have called CO2 insensitive 1 (cis1). BIG mutants are compromised in elevated CO2 -induced stomatal closure and bicarbonate activation of S-type anion channel currents. In contrast with the wild-type, they fail to exhibit reductions in stomatal density and index when grown in elevated CO2 . However, like the wild-type, BIG mutants display inhibition of stomatal opening when exposed to elevated CO2 . BIG mutants also display wild-type stomatal aperture responses to the closure-inducing stimulus abscisic acid (ABA). Our results indicate that BIG is a signaling component involved in the elevated CO2 -mediated control of stomatal development. In the control of stomatal aperture by CO2 , BIG is only required in elevated CO2 -induced closure and not in the inhibition of stomatal opening by this environmental signal. These data show that, at the molecular level, the CO2 -mediated inhibition of opening and promotion of stomatal closure signaling pathways are separable and BIG represents a distinguishing element in these two CO2 -mediated responses.

KEYWORDS:

Arabidopsis thaliana ; BIG gene; CO2 signaling; S-type anion channel; abscisic acid (ABA); stomatal function

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