Format

Send to

Choose Destination
Metallomics. 2018 Jan 24;10(1):73-76. doi: 10.1039/c7mt00285h.

Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite.

Author information

1
Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany. tanja.schwerdtle@uni-potsdam.de and NutriAct - Competence Cluster Nutrition Research Berlin-Potsdam, Germany.
2
Bundesanstalt für Materialforschung und - prüfung (BAM), Richard-Willstätter-Str. 11, 12489 Berlin, Germany.
3
Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany. tanja.schwerdtle@uni-potsdam.de.
4
Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany. tanja.schwerdtle@uni-potsdam.de and NutriAct - Competence Cluster Nutrition Research Berlin-Potsdam, Germany and Interactions of Essential Trace Elements in Healthy and Diseased Elderly (TraceAge), DFG Research Unit, FOR 2558, Nuthetal, Germany.

Abstract

Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 μM for 0-48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.

PMID:
29292446
DOI:
10.1039/c7mt00285h

Supplemental Content

Full text links

Icon for Royal Society of Chemistry
Loading ...
Support Center