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Comp Biochem Physiol B Biochem Mol Biol. 2018 Oct;224:88-97. doi: 10.1016/j.cbpb.2017.12.017. Epub 2017 Dec 27.

Metabolic suppression in the pelagic crab, Pleuroncodes planipes, in oxygen minimum zones.

Author information

1
College of Marine Science, University of South Florida, 830 1st St. S., St. Petersburg, FL 33701, USA. Electronic address: seibel@usf.edu.
2
Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada.
3
BioMEMS Resource Center & Center for Engineering in Medicine, Massachusetts General Hospital & Harvard Medical School, 114 16th Street, Charlestown, MA 02129, USA.
4
Evergreen State College, Olympia, WA, USA.

Abstract

The pelagic red crab, Pleuroncodes planipes, is abundant throughout the Eastern Tropical Pacific in both benthic and pelagic environments to depths of several hundred meters. The oxygen minimum zones in this region reaches oxygen levels as low as 0.1kPa at depths within the crabs vertical range. Crabs maintain aerobic metabolism to a critical PO2 of ~0.27±0.2kPa (10°C), in part by increasing ventilation as oxygen declines. At subcritical oxygen levels, they enhance anaerobic ATP production slightly as indicated by modest increases in lactate levels. However, hypoxia tolerance is primarily mediated via a pronounced suppression of aerobic metabolism (~70%). Metabolic suppression is achieved, primarily, via reduced protein synthesis, which is a major sink for metabolic energy. Posttranslational modifications on histone H3 suggest a condensed chromatin state and, hence, decreased transcription. Under hypoxia, p-H3S10, Ac-H3K9, Ac-H3K14 were 39, 68, and 36% of control values, respectively. We also report a net decrease in protein translation. In particular, eEF2 activity is reduced due to a ~5-fold increase in inhibitory phosphorylation and a significant decrease in protein level. Elevated heat shock proteins suggest that, despite impressive tolerance, the cellular stress response is triggered during hypoxia. We discuss the implications for pelagic ecology and biogeochemical cycles.

KEYWORDS:

Hypometabolism; Hypoxia; Protein synthesis; Vertical migration; Zooplankton

PMID:
29288744
DOI:
10.1016/j.cbpb.2017.12.017
[Indexed for MEDLINE]

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