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Food Chem. 2018 Apr 15;245:415-425. doi: 10.1016/j.foodchem.2017.10.114. Epub 2017 Oct 25.

Determination of free steroidal compounds in vegetable oils by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry.

Author information

1
Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China.
2
Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Laboratory of Quality and Safety Risk Assessment for Oilseed Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China; Quality Inspection and Test Center for Oilseed Products, Ministry of Agriculture, Wuhan 430062, China. Electronic address: zhanglx@caas.cn.
3
Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Laboratory of Quality and Safety Risk Assessment for Oilseed Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China; Quality Inspection and Test Center for Oilseed Products, Ministry of Agriculture, Wuhan 430062, China.
4
Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China.
5
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China.
6
Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Quality and Safety Risk Assessment for Oilseed Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China; Quality Inspection and Test Center for Oilseed Products, Ministry of Agriculture, Wuhan 430062, China. Electronic address: peiwuli@oilcrops.cn.

Abstract

A method based on comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC × GC-TOF/MS) was developed to analyze steroidal compounds in vegetable oils, which could provide better separation and higher sensitivity than conventional one dimensional gas chromatography, and allowed determination of 31 sterols and triterpene alcohols in one injection. Furthermore, the approach also permitted separation and detection of small amounts of other compounds (may be steroidal compounds whose molecular structures have not been confirmed), which were obscured in the lower-resolution single-column technique. With the help of the GC × GC system, a more elaborate and complete information regarding the distributions and concentrations of free phytosterols and triterpene alcohols in safflower seed oil, soybean oil, rapeseed oil, sunflower seed oil and peanut oil were obtained. The proposed method could potentially open a new opportunity for the more in-depth knowledge of the steroidal compounds of vegetable oils.

KEYWORDS:

Analysis; Free steroidal compounds; GC × GC-TOF/MS; Vegetable oils

PMID:
29287390
DOI:
10.1016/j.foodchem.2017.10.114
[Indexed for MEDLINE]

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