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ACS Nano. 2018 Jan 23;12(1):494-503. doi: 10.1021/acsnano.7b07060. Epub 2018 Jan 4.

Multiplexed Profiling of Single Extracellular Vesicles.

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Center for Systems Biology, Massachusetts General Hospital , 185 Cambridge St, CPZN 5206, Boston, Massachusetts 02114, United States.
Department of Neurology, Massachusetts General Hospital , Boston, Massachusetts 02114, United States.
Department of Neurosurgery, Brigham and Women's Hospital , Boston, Massachusetts 02115, United States.
Department of Systems Biology, Harvard Medical School , 200 Longwood Ave, Boston, Massachusetts 02115, United States.


Extracellular vesicles (EV) are a family of cell-originating, membrane-enveloped nanoparticles with diverse biological function, diagnostic potential, and therapeutic applications. While EV can be abundant in circulation, their small size (∼4 order of magnitude smaller than cells) has necessitated bulk analyses, making many more nuanced biological explorations, cell of origin questions, or heterogeneity investigations impossible. Here we describe a single EV analysis (SEA) technique which is simple, sensitive, multiplexable, and practical. We profiled glioblastoma EV and discovered surprising variations in putative pan-EV as well as tumor cell markers on EV. These analyses shed light on the heterogeneous biomarker profiles of EV. The SEA technology has the potential to address fundamental questions in vesicle biology and clinical applications.


cancer; diagnostic; exosomes; extracellular vesicle; imaging

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