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Anal Chem. 2018 Feb 6;90(3):1601-1607. doi: 10.1021/acs.analchem.7b02494. Epub 2018 Jan 22.

Electrochemical Investigation of the Interaction between Catecholamines and ATP.

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Department of Chemistry and Chemical Engineering, Chalmers University of Technology , 41296 Gothenburg, Sweden.
Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna , 38200 San Cristóbal de La Laguna, Santa Cruz de Tenerife, Spain.
Department of Chemistry and Chemical Biology, University of Gothenburg , 41296 Gothenburg, Sweden.
Instituto Universitario de BioOrgánica, Universidad de La Laguna , 38200 San Cristóbal de La Laguna, Santa Cruz de Tenerife, Spain.


The study of the colligative properties of adenosine 5'-triphosphate (ATP) and catecholamines has received the attention of scientists for decades, as they could explain the capabilities of secretory vesicles (SVs) to accumulate neurotransmitters. In this Article, we have applied electrochemical methods to detect such interactions in vitro, at the acidic pH of SVs (pH 5.5) and examined the effect of compounds having structural similarities that correlate with functional groups of ATP (adenosine, phosphoric acid and sodium phosphate salts) and catecholamines (catechol). Chronoamperometry and fast scan cyclic voltammetry (FSCV) provide evidence compatible with an interaction of the catechol and adenine rings. This interaction is also reinforced by an electrostatic interaction between the phosphate group of ATP and the protonated ammonium group of catecholamines. Furthermore, chronoamperometry data suggest that the presence of ATP subtlety reduces the apparent diffusion coefficient of epinephrine in aqueous media that adds an additional factor leading to a slower rate of catecholamine exocytosis. This adds another plausible mechanism to regulate individual exocytosis events to alter communication.

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