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Exp Ther Med. 2017 Dec;14(6):5589-5596. doi: 10.3892/etm.2017.5254. Epub 2017 Oct 3.

miR-34a and miR-125b are upregulated in peripheral blood mononuclear cells from patients with type 2 diabetes mellitus.

Author information

1
Department of Laboratory Medicine, The Second People's Hospital of Taicang, Taicang, Jiangsu 215400, P.R. China.
2
Department of Immunology, Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China.
3
Department of Laboratory Medicine, Shanghai Gongli Hospital, The Second Military Medical University, Shanghai 200135, P.R. China.
4
Department of Laboratory Medicine, The People's Hospital of Taicang, Taicang, Jiangsu 215400, P.R. China.

Abstract

Type 2 diabetes mellitus (T2DM) is a leading cause of blindness, non-traumatic amputation and end-stage renal disease, as well as a major cardiovascular risk factor. To determine whether miR-125b and miR-34a serve an important role in the development of T2DM, the current study investigated the expression profile of two microRNAs (miR-34a and miR-125b) and their relative genes in peripheral blood mononuclear cells from 73 patients with T2DM and 52 healthy donors by reverse transcription-quantitative polymerase chain reaction In addition, the association between miR-34a, miR-125b and their relevant genes expression profile were analyzed with respect to the pathogenesis of T2DM. The present study demonstrated that the expression levels of miR-125b and miR-34a were elevated in peripheral blood mononuclear cell samples from patients with T2DM. Furthermore, miR-34a and miR-125b were positively correlated with low-density lipoprotein/high-density lipoprotein (HDL) and Foxp3 and negatively related to triglyceride/HDL. However, no correlation among miR-34a, miR-125b and the value of homeostasis model assessment of insulin resistance, homeostasis model assessment of β-cell function and the genes of B lymphocyte-induced maturation protein-1, interferon regulatory factor-4, P53 and retinoid-related orphan receptor γt were observed. These results indicate that the alteration of miR-34a and miR-125b exists in patients with T2DM, which may be involved in the pathogenesis of T2DM, and could be a potential novel biomarker of T2DM.

KEYWORDS:

B lymphocyte-induced maturation protein-1; P53; forkhead box protein 3; interferon regulatory protein-4 transcription factors; miR-125b; miR-34a; peripheral blood mononuclear cells; retinoid-related orphan receptor γt; type 2 diabetes mellitus

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