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Exp Ther Med. 2017 Dec;14(6):5589-5596. doi: 10.3892/etm.2017.5254. Epub 2017 Oct 3.

miR-34a and miR-125b are upregulated in peripheral blood mononuclear cells from patients with type 2 diabetes mellitus.

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Department of Laboratory Medicine, The Second People's Hospital of Taicang, Taicang, Jiangsu 215400, P.R. China.
Department of Immunology, Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China.
Department of Laboratory Medicine, Shanghai Gongli Hospital, The Second Military Medical University, Shanghai 200135, P.R. China.
Department of Laboratory Medicine, The People's Hospital of Taicang, Taicang, Jiangsu 215400, P.R. China.


Type 2 diabetes mellitus (T2DM) is a leading cause of blindness, non-traumatic amputation and end-stage renal disease, as well as a major cardiovascular risk factor. To determine whether miR-125b and miR-34a serve an important role in the development of T2DM, the current study investigated the expression profile of two microRNAs (miR-34a and miR-125b) and their relative genes in peripheral blood mononuclear cells from 73 patients with T2DM and 52 healthy donors by reverse transcription-quantitative polymerase chain reaction In addition, the association between miR-34a, miR-125b and their relevant genes expression profile were analyzed with respect to the pathogenesis of T2DM. The present study demonstrated that the expression levels of miR-125b and miR-34a were elevated in peripheral blood mononuclear cell samples from patients with T2DM. Furthermore, miR-34a and miR-125b were positively correlated with low-density lipoprotein/high-density lipoprotein (HDL) and Foxp3 and negatively related to triglyceride/HDL. However, no correlation among miR-34a, miR-125b and the value of homeostasis model assessment of insulin resistance, homeostasis model assessment of β-cell function and the genes of B lymphocyte-induced maturation protein-1, interferon regulatory factor-4, P53 and retinoid-related orphan receptor γt were observed. These results indicate that the alteration of miR-34a and miR-125b exists in patients with T2DM, which may be involved in the pathogenesis of T2DM, and could be a potential novel biomarker of T2DM.


B lymphocyte-induced maturation protein-1; P53; forkhead box protein 3; interferon regulatory protein-4 transcription factors; miR-125b; miR-34a; peripheral blood mononuclear cells; retinoid-related orphan receptor γt; type 2 diabetes mellitus

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