Format

Send to

Choose Destination
Molecules. 2017 Dec 28;23(1). pii: E66. doi: 10.3390/molecules23010066.

Activation Profile Analysis of CruCA4, an α-Carbonic Anhydrase Involved in Skeleton Formation of the Mediterranean Red Coral, Corallium rubrum.

Author information

1
Istituto di Bioscienze e Biorisorse, CNR, Via Pietro Castellino 111, 80131 Napoli, Italy. sonia.delprete@ibbr.cnr.it.
2
Dipartimento Di Chimica, Laboratorio di Chimica Bioinorganica, Università degli Studi di Firenze, Polo Scientifico, Via della Lastruccia 3, 50019 Sesto Fiorentino (Florence), Italy. daniela.vullo@unifi.it.
3
Centre Scientifique de Monaco, 8 Quai Antoine 1°, 98000 Monaco, Monaco. zoccola@centrescientifique.mc.
4
Centre Scientifique de Monaco, 8 Quai Antoine 1°, 98000 Monaco, Monaco. stambutte@centrescientifique.mc.
5
Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche, Università degli Studi di Firenze, Via U. Schiff 6, 50019 Sesto Fiorentino (Florence), Italy. claudiu.supuran@unifi.it.
6
Istituto di Bioscienze e Biorisorse, CNR, Via Pietro Castellino 111, 80131 Napoli, Italy. clemente.capasso@ibbr.cnr.it.

Abstract

CruCA4, a coral α-carbonic anhydrase (CA, EC 4.2.1.1) involved in the biomineralization process of the Mediterranean red coral, Corallium rubrum, was investigated for its activation with a panel of amino acids and amines. Most compounds showed considerable activating properties, with a rather well defined structure-activity relationship. The most effective CruCA4 activators were d-His, 4-H₂N-l-Phe, Histamine, Dopamine, Serotonin, 1-(2-Aminoethyl)-piperazine, and l-Adrenaline, with activation constants in the range of 8-98 nM. Other amines and amino acids, such as d-DOPA, l-Tyr, 2-Pyridyl-methylamine, 2-(2-Aminoethyl) pyridine and 4-(2-Aminoethyl)-morpholine, were submicromolar CruCA4 activators, with KA ranging between 0.15 and 0.93 µM. Since it has been shown that CA activators may facilitate the initial phases of in-bone mineralization, our study may be relevant for finding modulators of enzyme activity, which can enhance the formation of the red coral skeleton.

KEYWORDS:

activators; amine; amino acid; biomineralization; calcification; carbonic anhydrase; coral

PMID:
29283417
PMCID:
PMC6017236
DOI:
10.3390/molecules23010066
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center