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Chronobiol Int. 2018 Jan;35(1):132-136. doi: 10.1080/07420528.2017.1316731. Epub 2017 Dec 28.

Circadian PER2 protein oscillations do not persist in cycloheximide-treated mouse embryonic fibroblasts in culture.

Author information

1
a Department of Systems Biology, Graduate School of Pharmaceutical Sciences , Kyoto University , Kyoto , Japan.

Abstract

It is not known whether the endogenous mammalian core clock proteins sustain measurable oscillations in cells in culture where de novo translation is pharmacologically inhibited. We studied here the mammalian core clock protein PER2, which undergoes robust circadian oscillations in both abundance and phosphorylation. With a newly developed antibody that enables tracing the endogenous PER2 protein oscillations over circadian cycles with cultured mouse embryonic fibroblast cells, we provide evidence that PER2 does not persist noticeable circadian rhythms when translation is inhibited.

KEYWORDS:

PER2; antibody; clock protein; cycloheximide

PMID:
29283306
DOI:
10.1080/07420528.2017.1316731
[Indexed for MEDLINE]

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