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Cell Rep. 2017 Dec 26;21(13):3970-3986. doi: 10.1016/j.celrep.2017.12.017.

Developmental Emergence of Adult Neural Stem Cells as Revealed by Single-Cell Transcriptional Profiling.

Author information

1
Program in Neuroscience and Mental Health, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada.
2
Program in Neuroscience and Mental Health, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada; Institute of Medical Science , University of Toronto, Toronto, ON M5G 1A8, Canada.
3
The Donnelly Centre , University of Toronto, Toronto, ON M5G 1A8, Canada; Department of Molecular Genetics , University of Toronto, Toronto, ON M5G 1A8, Canada.
4
Princess Margaret Genomics Centre, University Health Network, Toronto, ON M5G 2M9, Canada.
5
Program in Neuroscience and Mental Health, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada; Institute of Medical Science , University of Toronto, Toronto, ON M5G 1A8, Canada; Department of Molecular Genetics , University of Toronto, Toronto, ON M5G 1A8, Canada.
6
Program in Neuroscience and Mental Health, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada; Institute of Medical Science , University of Toronto, Toronto, ON M5G 1A8, Canada; Department of Molecular Genetics , University of Toronto, Toronto, ON M5G 1A8, Canada; Department of Physiology, University of Toronto, Toronto, ON M5G 1A8, Canada. Electronic address: fredam@sickkids.ca.

Abstract

Adult neural stem cells (NSCs) derive from embryonic precursors, but little is known about how or when this occurs. We have addressed this issue using single-cell RNA sequencing at multiple developmental time points to analyze the embryonic murine cortex, one source of adult forebrain NSCs. We computationally identify all major cortical cell types, including the embryonic radial precursors (RPs) that generate adult NSCs. We define the initial emergence of RPs from neuroepithelial stem cells at E11.5. We show that, by E13.5, RPs express a transcriptional identity that is maintained and reinforced throughout their transition to a non-proliferative state between E15.5 and E17.5. These slowly proliferating late embryonic RPs share a core transcriptional phenotype with quiescent adult forebrain NSCs. Together, these findings support a model wherein cortical RPs maintain a core transcriptional identity from embryogenesis through to adulthood and wherein the transition to a quiescent adult NSC occurs during late neurogenesis.

KEYWORDS:

Drop-seq; cortex; development; intermediate progenitors; neural stem cells; neurogenesis; radial precursors; single-cell RNA-seq

PMID:
29281841
DOI:
10.1016/j.celrep.2017.12.017
[Indexed for MEDLINE]
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