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J Environ Sci Health B. 2018 Apr 3;53(4):255-260. doi: 10.1080/03601234.2017.1410416. Epub 2017 Dec 26.

High-sensitivity direct analysis of aflatoxins in peanuts and cereal matrices by ultra-performance liquid chromatography with fluorescence detection involving a large volume flow cell.

Author information

1
a National Referral Laboratory , ICAR-National Research Centre for Grapes, Manjri Farm , Pune , Maharashtra , India.

Abstract

This paper reports a sensitive and cost effective method of analysis for aflatoxins B1, B2, G1 and G2. The sample preparation method was primarily optimised in peanuts, followed by its validation in a range of peanut-processed products and cereal (rice, corn, millets) matrices. Peanut slurry [12.5 g peanut + 12.5 mL water] was extracted with methanol: water (8:2, 100 mL), cleaned through an immunoaffinity column and thereafter measured directly by ultra-performance liquid chromatography-fluorescence (UPLC-FLD) detection, within a chromatographic runtime of 5 minutes. The use of a large volume flow cell in the FLD nullified the requirement of any post-column derivatisation and provided the lowest ever reported limits of quantification of 0.025 for B1 and G1 and 0.01 μg/kg for B2 and G2. The single laboratory validation of the method provided acceptable selectivity, linearity, recovery and precision for reliable quantifications in all the test matrices as well as demonstrated compliance with the EC 401/2006 guidelines for analytical quality control of aflatoxins in foodstuffs.

KEYWORDS:

Aflatoxins; UPLC; cereals; fluorescence detection; peanut-processed products; peanuts

PMID:
29278977
DOI:
10.1080/03601234.2017.1410416
[Indexed for MEDLINE]

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