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Physiol Int. 2017 Dec 1;104(4):291-300. doi: 10.1556/2060.104.2017.4.2.

Effect of α-tocopherol megadoses on hematologic parameters and antioxidant capacity of rats in an ultraendurance probe.

Author information

1 Applied Psychophysiological Research Group, European University of Madrid , Madrid, Spain.
2 Infine Research Group, Department of Health and Sport Performance, INEF, Polytechnic University of Madrid , Madrid, Spain.
3 Department of Physiology, Biomedical Research Center, Institute of Nutrition and Food Technology "Jose Mataix", University of Granada , Granada, Spain.
4 Faculty of Pharmacy, Department of Physiology, University of Granada , Granada, Spain.


This study was aimed to analyze the effect of two different megadoses of α-tocopherol (vit E) in the antioxidant activity and red and white blood series of Wistar rats after a 180-min ultraendurance probe. Three groups of 10 rats were analyzed; VEAG: acute administration of a megadoses of 5,000 IU/kg of vit E the day before the probe; VECG: chronic administration of 1,000 IU/kg/day of vit E for 6 days before the probe; CG: placebo administration. VEAG presented white cells, red blood cells, hematocrit, hemoglobin values significantly higher than CG and VECG (p < 0.05). The mean corpuscular hemoglobin and lymphocytes concentrations were significantly higher in the VECG than in the other two groups (p < 0.05). Similarly, VEAG presented a significantly higher vit E blood concentration than VECG and CG (p < 0.05), and VECG than CG (p < 0.05). Finally, we found a significantly positive correlation between trolox equivalent antioxidant capacity (TEAC) and red blood cells concentration (r = 0.374) and a significantly inverse correlation between TEAC and blood lactate concentration (r = -0.365). Our findings suggest that acute vit E megadoses could protect against transitory sport anemia symptoms and increase the white blood cell count in comparison with the chronic dose and control groups after an ultraendurance probe.


antioxidant capacity; red blood cell; running performance; sport anemia; ultraendurance; vitamin E; white blood cell; wistar rat

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