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Bioresour Technol. 2018 Mar;251:204-209. doi: 10.1016/j.biortech.2017.12.052. Epub 2017 Dec 18.

A simple method for assaying anaerobic biodegradation of dyes.

Author information

1
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China; CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science & Technology of China, Hefei 230026, China.
2
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
3
CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science & Technology of China, Hefei 230026, China.
4
CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science & Technology of China, Hefei 230026, China. Electronic address: hqyu@ustc.edu.cn.

Abstract

Anaerobic dye degradation is usually assayed using serum vials, which is time-consuming and costly. In this work, a simple method was established for real-time nondestructive assay of dye biodegradation using 96-well microtiter plates with petrolatum oil to avoid the volatilization and high transmittance transparent tape to prevent the permeation of oxygen. With the anaerobic degradation of methyl red and amaranth by Shewanella oneidensis MR-1, this assay method was verified. Further experiments revealed that blocking Mtr pathway had no substantial effect on the degradation of methyl red and dose of riboflavin also failed to promote the degradation of methyl red. On the contrary, the anaerobic degradation of amaranth depended mainly on the electron transmembrane transfer through Mtr pathway. Our work clearly indicates that Mtr pathway had different effects on intra- and extra-cellular degradation of azo dyes by S. oneidensis MR-1. Such a developed method is helpful for investigating anaerobic dye decolorization.

KEYWORDS:

Anaerobic assay; Dye degradation; High-throughput method; Shewanella oneidensis MR-1

PMID:
29277051
DOI:
10.1016/j.biortech.2017.12.052
[Indexed for MEDLINE]

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