R-ISC Numbers Increase and Enter the Cell Cycle following αCD3
(A) mTert-GFP+ crypt cells per 150 crypt sections at baseline, injury, early recovery, and late recovery. N = 2 mice/time point, 18, 21, 20, and 9 sections counted.
(B) mTert-GFP+ crypt cells co-expressing Ki67 in control IgG and αCD3-treated mice at early recovery. Representative optical sections from control IgG- (N = 8) and αCD3-treated (N = 4) mice showing cycling (arrowhead) and quiescent (arrow) mTert-GFP+ cells in merged, DAPI, mTert-GFP, and Ki67 channels.
(C) mTert-GFP+ cells co-expressing EdU in isolated crypts from control IgG- (N = 3) and αCD3-treated (N = 3) mice at early recovery. Representative optical sections showing quiescent mTert-GFP+ cells (arrow) in control IgG and cycling mTert-GFP+ cells in αCD3-treated (arrowhead) crypts in merged DAPI, mTert-GFP, and EdU (Click-iT) channels.
Error bars indicate the SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Scale bar, 10 μm.