Format

Send to

Choose Destination
Anim Reprod Sci. 2018 Feb;189:30-42. doi: 10.1016/j.anireprosci.2017.12.004. Epub 2017 Dec 16.

Rabbit seminal plasma proteome: The importance of the genetic origin.

Author information

1
Animal Technology and Research Center (CITA), Instituto Valenciano de Investigaciones Agrarias (IVIA), Polígono La Esperanza n° 100, 12400 Segorbe, Castellón, Spain.
2
Institute of Science and Animal Technology (ICTA), Universitat Politècnica de València, 46022 Valencia, Spain.
3
Animal Technology and Research Center (CITA), Instituto Valenciano de Investigaciones Agrarias (IVIA), Polígono La Esperanza n° 100, 12400 Segorbe, Castellón, Spain. Electronic address: viudes_mar@gva.es.

Abstract

The present study was conducted to characterise rabbit seminal plasma proteins (SP proteins) focusing on the influence of the genetic origin and seasonality. In addition, β-NGF protein quantity in SP was determined. Semen samples were recovered from January to December 2014 using 6 males belonging to genotype A and six from genotype R. For each genotype, one pooled sample at the beginning, middle and end of each season was selected to develop the experiment. A total of 24 pools (3 for each season and genetic line) were analysed. SP proteins of the two experimental groups were recovered and subjected to in-solution digestion nano LC-MS/MS and bioinformatics analysis. The resulting library included 402 identified proteins validated with ≥95% Confidence (unused Score ≥ 1.3). These data are available via ProteomeXchange with identifier PXD006308. Only 6 proteins were specifically implicated in reproductive processes according to Gene Ontology annotation. Twenty-three proteins were differentially expressed between genotypes, 11 over-expressed in genotype A and 12 in genotype R. Regarding the effect of season on rabbit SP proteome, results showed that there is no clear pattern of protein variation throughout the year. Similar β-NGF relative quantity was observed between seasons and genotypes. In conclusion, this study generates the largest library of SP proteins reported to date in rabbits and provides evidence that genotype is related to a specific abundance of SP proteins.

KEYWORDS:

Genotype; LC–MS/MS; Proteome; Rabbit; Season; Seminal plasma

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center