Format

Send to

Choose Destination
Sci Rep. 2017 Dec 22;7(1):18098. doi: 10.1038/s41598-017-17780-6.

Elimination of Antibiotic Resistant Surgical Implant Biofilms Using an Engineered Cationic Amphipathic Peptide WLBU2.

Author information

1
Arthritis and Arthroplasty Design Group, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
2
Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA, USA.
3
Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.
4
Division of Infectious Diseases, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
5
Arthritis and Arthroplasty Design Group, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA. urishk2@upmc.edu.
6
The Magee Bone and Joint Center, University of Pittsburgh Medical Center, Pittsburgh, PA, USA. urishk2@upmc.edu.
7
Clinical & Translational Science Institute, University of Pittsburgh, Pittsburgh, PA, USA. urishk2@upmc.edu.
8
Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA. urishk2@upmc.edu.
9
Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA. urishk2@upmc.edu.

Abstract

Antibiotics are unable to remove biofilms from surgical implants. This high antibiotic tolerance is related to bacterial persisters, a sub-population of bacteria phenotypically tolerant to antibiotics secondary to a reduced metabolic state. WLBU2 is an engineered cationic amphipathic peptide designed to maximize antimicrobial activity with minimal mammalian cell toxicity. The objective of this study was to test the ability of WLBU2 to remove Staphylococcus aureus surgical implant biofilms. WLBU2 effectively treated S. aureus biofilms formed by a variety of clinical MSSA and MRSA strains and created culture-negative implants in the in vitro biofilm model. Blocking bacterial metabolism by inhibiting oxidative phosphorylation did not affect WLBU2 killing compared to decreased killing by cefazolin. In the surgical implant infection animal model, WLBU2 decreased biofilm mass as compared to control, untreated samples. WLBU2 could rapidly eliminate implants in vitro and had sufficient efficacy in vivo with minimal systemic toxicity.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center