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Sci Rep. 2017 Dec 22;7(1):18050. doi: 10.1038/s41598-017-18374-y.

Resequencing of the Leishmania infantum (strain JPCM5) genome and de novo assembly into 36 contigs.

Author information

1
Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Campus de Excelencia Internacional (CEI) UAM+CSIC, Universidad Autónoma de Madrid, Madrid, Spain.
2
World Health Organization Collaborating Centre for Leishmaniasis, Laboratory of Reference and Research in Parasitology, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Madrid, Spain.
3
Red de Investigación Colaborativa en Enfermedades Tropicales (RICET), ISCIII, Madrid, Spain.
4
Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Campus de Excelencia Internacional (CEI) UAM+CSIC, Universidad Autónoma de Madrid, Madrid, Spain. jmrequena@cbm.csic.es.
5
Red de Investigación Colaborativa en Enfermedades Tropicales (RICET), ISCIII, Madrid, Spain. jmrequena@cbm.csic.es.
6
Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Campus de Excelencia Internacional (CEI) UAM+CSIC, Universidad Autónoma de Madrid, Madrid, Spain. baguado@cbm.csic.es.
7
Red de Investigación Colaborativa en Enfermedades Tropicales (RICET), ISCIII, Madrid, Spain. baguado@cbm.csic.es.

Abstract

Leishmania parasites are the causative of leishmaniasis, a group of potentially fatal human diseases. Control strategies for leishmaniasis can be enhanced by genome based investigations. The publication in 2005 of the Leishmania major genome sequence, and two years later the genomes for the species Leishmania braziliensis and Leishmania infantum were major milestones. Since then, the L. infantum genome, although highly fragmented and incomplete, has been used widely as the reference genome to address whole transcriptomics and proteomics studies. Here, we report the sequencing of the L. infantum genome by two NGS methodologies and, as a result, the complete genome assembly on 36 contigs (chromosomes). Regarding the present L. infantum genome-draft, 495 new genes have been annotated, a hundred have been corrected and 75 previous annotated genes have been discontinued. These changes are not only the result of an increase in the genome size, but a significant contribution derives from the existence of a large number of incorrectly assembled regions in current chromosomal scaffolds. Furthermore, an improved assembly of tandemly repeated genes has been obtained. All these analyses support that the de novo assembled L. infantum genome represents a robust assembly and should replace the currently available in the databases.

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