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Cell Mol Biol Lett. 2017 Dec 19;22:29. doi: 10.1186/s11658-017-0055-z. eCollection 2017.

The role of bone marrow mononuclear cell-conditioned medium in the proliferation and migration of human dermal fibroblasts.

Author information

1
Unidad de Coordinación de Trasplantes, Terapia Celular y Medicina Regenerativa, Hospital Universitario Central de Asturias, Oviedo, Spain.
2
Departamento de Morfología y Biología Celular, Universidad de Oviedo, Oviedo, Spain.
3
Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Temuco, Chile.
#
Contributed equally

Abstract

Background:

Several recent studies have demonstrated the great potential of bone marrow cells in regenerative medicine, not only for their ability to differentiate to match a damaged cell type, but also because they synthesize and release various growth factors and cytokines.We examined the effect of bone marrow cell-conditioned medium in the healing process, especially in terms of fibroblast proliferation and migration.

Methods:

These in vitro studies consisted of co-culture (without direct contact) of dermal fibroblasts with mononuclear bone marrow cells and the use of conditioned medium obtained from these cultures in a scratch wound model.

Results:

Mononuclear cells were found to increase the proliferation of fibroblasts, and the conditioned medium showed a stimulatory effect on the migration of fibroblasts.

Conclusion:

When considered together with the observed increase in growth factor levels in conditioned medium, it appears that these cells act through a paracrine mechanism.

KEYWORDS:

Bone marrow mononuclear cells; Cell migration and proliferation; Human dermal fibroblasts; Paracrine interactions; Wound repair

PMID:
29270201
PMCID:
PMC5735620
DOI:
10.1186/s11658-017-0055-z
[Indexed for MEDLINE]
Free PMC Article

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