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Methods. 2018 Feb 1;134-135:50-55. doi: 10.1016/j.ymeth.2017.12.013. Epub 2017 Dec 19.

Cell sorting of various cell types from mouse and human skeletal muscle.

Author information

1
Institut Cochin, INSERM U1016, CNRS UMR8104, Université Paris Descartes, PRES Sorbonne-Paris-Cité, Paris, France.
2
Institut NeuroMyoGène, Univ Lyon, Université Claude Bernard Lyon 1, INSERM U1217, CNRS UMR5310, Lyon, France.
3
Institut NeuroMyoGène, Univ Lyon, Université Claude Bernard Lyon 1, INSERM U1217, CNRS UMR5310, Lyon, France. Electronic address: benedicte.chazaud@inserm.fr.

Abstract

Muscle stem cells or satellite cells are required for skeletal muscle regeneration. It has been shown that the satellite cell microenvironment, including neighboring cells such as endothelial cells, macrophages or fibroblasts are essential for complete and efficient regeneration. A deficient behavior of these cells compromises regeneration. Therefore, there is a strong interest in understanding the cellular and molecular interactions at work between these cell types during muscle regeneration. Fluorescence-activated cell sorting allows to isolate these four cell types at different time points of regeneration, for further high throughput or behavioral experiments. We present here a method for the concomitant isolation of 4 cell types present in the regenerating skeletal muscle: muscle stem cells, endothelial cells, fibro-adipogenic precursor cells and macrophages.

KEYWORDS:

Cell sorting; Endothelial cells; Fibroblasts; Macrophages; Myogenic cells; Skeletal muscle regeneration

PMID:
29269151
DOI:
10.1016/j.ymeth.2017.12.013
[Indexed for MEDLINE]

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