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Methods Mol Biol. 2018;1722:249-260. doi: 10.1007/978-1-4939-7553-2_16.

Extracellular Matrix Molecule-Based Capture of Mesenchymal Stromal Cells Under Flow.

Author information

1
Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Biology, Medicine and Health, University of Manchester, Manchester, UK.
2
Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Biology, Medicine and Health, University of Manchester, Manchester, UK. cay.kielty@manchester.ac.uk.

Abstract

We present a method to capture mesenchymal stromal cells (MSCs) by adhesion to extracellular matrix (ECM) molecules under flow conditions. The technique simulates a physiological system and exploits the natural biological interactions of cells, through integrin receptors, with their ECM. The system offers an insight into how MSCs could be targeted/localized to the site of interest (graft) following intravenous injection.

KEYWORDS:

Adhesion; Fibronectin; Laminin; Mesenchymal stromal cell

PMID:
29264810
DOI:
10.1007/978-1-4939-7553-2_16
[Indexed for MEDLINE]

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