[Gene Polymorphism 24 Rare Blood Group Antigens of 11 RBC Blood Group Systems in Manchu Population of Harbin Area in China]

Objective: The purpose of this study was to investigate the 24 kinds of antigen gene polymorphism chara-cteristics in 11 RBC blood group systems of Manchu population blood donor in Harbin Area of China, so as to better perform the clinical blood transfusion and to avoid adverse transfusion reactions.

Methods: Twenty-four antigen genes (GYPB (S/S), Duffy, Kell, Dombrock, Diego, Kidd, Scianna, Colton, Lutheran, Yt, Mur) were assessed by the polymerase chain reaction sequence specific primers (PCR-SSP) in 11 blood type systems, including Fy^a, Fy^b; K, K; Do^a, Do^b; Di^a, Di^b; JK^a, JK^b; Sc1,Sc2; Co^a, Co^b; Lu^a, Lu^b; Yt^a, Yt^b; Kp^a, Kp^b; Mur, Mur2 among 200 Manchus in Harbin.

Results: The gene frequency of blood type system in Harbin Manchu GYPB (S/S) was as follows: S=0.0625, s=0.9325; the gene frequency of duffy blood type system: Fy^a=0.0525, Fy^b=0.9475; Gene frequency of Dombrock type system was as follows: Do^a=0.1250, Do^b=0.8750; Gene frequency of Diego type system: Di^a=0.0275, Di^b=0.9725; the gene frequency of Kidd blood type system: JK^a=0.5650, JK^b=0.4350; the gene frequency of Mur blood type system: Mur1 = 0.0050, Mur2 = 0.9950; the Kell, Scianna, Colton, Lutheran and Yt type blood type system displayed a monomorph distribution and the genotype was kkKp^bKp^b,Sc1Sc1,Co^aCo^a, Lu^bLu^b, Yt^aYt^a, respectively.

Conclusion: The distribution of GYPB(S/s),Duffy,Dombrock, Diego, Kidd and Mur blood type system in Harbin Manchu has been found to be characterized by polymorphism, and the distribution of kell, scianna, colton, lutheran and ye blood type system has been found to be characterized by oligomorphism. The gene destribution of dombrock and Kidd blood group systems is unique in Harbin Manchu.